Bovine serum albumin (BSA), fibrinogen from human plasma (FBG), GRGDSP peptide, collagen type I from rat tail, acetic acid, eriochrome black T, phosphate buffered saline (PBS), and sodium hydroxide (NaOH) were purchased from Sigma-Aldrich (St. Louis, MO). Alpha-minimum essential medium (α-MEM) with nucleosides, calcium chloride dihydrate (CaCl2●2H20), fetal bovine serum (FBS), sodium chloride (NaCl), ethylenediaminetetraacetic acid (EDTA), and a live/dead cytotoxicity kit for mammalian cells were purchased from Thermo Fisher Scientific (Hampton, NH). Penicillin-streptomycin, tris hydrochloride, trypan blue, trypsin (0.25%) EDTA (1x), trypsin soybean inhibitor, paraformaldehyde, hematoxylin, and potassium chloride (KCl) were purchased from VWR (Radnor, PA). BTT 3033 (1-(4-Fluorophenyl)-N-methyl-N-[4[[(phenylamino)carbonyl]amino]phenyl]-1H-pyrazole-4-sulfonamide) was purchased from R&D Systems (Minneapolis, MN). MC3T3-E1 subclone 14 cells (batch number 61723894) were purchased from the American Type Culture Collection (ATCC; CRL-2594) (Manassas, VA).
Live dead cytotoxicity kit for mammalian cells
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Live/Dead Cytotoxicity Kit for Mammalian Cells is a fluorescence-based assay designed to quantitatively measure cell viability and cytotoxicity. The kit utilizes a two-color fluorometric staining procedure to identify live (green) and dead (red) cells simultaneously.
Automatically generated - may contain errors
Lab products found in correlation
Fibrinogen from human plasma, by Merck Group (1 mentions)
Hematoxylin, by Avantor (1 mentions)
Trypan blue, by Avantor (1 mentions)
Penicillin streptomycin, by Avantor (1 mentions)
Collagen type 1 from rat tail, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Paraformaldehyde (pfa), by Avantor (1 mentions)
Eriochrome black t, by Merck Group (1 mentions)
Tris hydrochloride, by Avantor (1 mentions)
Grgdsp peptide, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Inverted microscope, by Nikon (1 mentions)
2 protocols using live dead cytotoxicity kit for mammalian cells
1
MC3T3-E1 Cell Culture and Characterization
2
Evaluating Primary Myoblast Viability
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To determine the cell viability of cultured primary myoblasts in microfluidic devices a cell viability assay was performed using the LIVE/DEAD cytotoxicity kit for mammalian cells according to manufacturer’s protocol (Thermofisher, L3324, Waltham, MA, USA). Briefly, 90,000 cells were seeded in either microfluidic devices or glass bottom 24 well plates (control) and maintained for 48 h. Calcein AM and ethidium homodimer were reconstituted in DMEM with GlutaMAX at 2 µM and 4 µM, respectively. Fluorescent imaging was performed using a 10X objective on an inverted microscope (Nikon, Japan). Image analysis and cell counts were performed in ImageJ (NIH, Bethesda, MD, USA).
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