Alexa fluor 594 goat anti rabbit igg h l a11012

Manufactured by Thermo Fisher Scientific

Alexa Fluor™ 594 goat anti-rabbit IgG(H + L) A11012 is a fluorescently-labeled secondary antibody used for detection and visualization of rabbit primary antibodies in various immunoassay techniques. It is conjugated with the Alexa Fluor 594 dye, which has excitation and emission maxima of 590 nm and 617 nm, respectively.

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Lab products found in correlation

Alexa fluor 488 goat anti mouse igg h l a11001, by Thermo Fisher Scientific (2 mentions) Tubb3, by BioLegend (1 mentions) Goat anti mouse igg hrp, by Proteintech (1 mentions) Sc 8017, by Santa Cruz Biotechnology (1 mentions) Gapdh 60004 1 ig, by Proteintech (1 mentions)

2 protocols using alexa fluor 594 goat anti rabbit igg h l a11012

Immunofluorescence Staining of Cytokine Receptors

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After 24 h of cytokine/inflammatory mediator treatment, cells were fixated (4% in paraformaldehyde (PFA) in phosphate-buffered saline (PBS)) and permeabilized (0.2% Titron X-100 in PBS; ThermoFisher Scientific) for immunofluorescence staining. Monoclonal antibodies against Class III ß-tubulin (TUBB3; 657,402, BioLegend), microtubule-associated protein 2 (MAP2; sc-74421, Santa Cruz), IL-17RA (clone G9, sc-376374, Santa Cruz), IL-10RB (clone F6, sc-271969, Santa Cruz), TNFR1 (clone H-5, sc-8436 Santa Cruz) or IFNGR1 (clone GIR 94, sc-12755, Santa Cruz) were added, and cells were incubated for 1 h at room temperature. Afterwards, matching secondary antibodies (Alexa Fluor™ 594 goat anti-mouse IgG(H + L) A11032, Alexa Fluor™ 488 goat anti-mouse IgG(H + L) A11001, Alexa Fluor™ 488 donkey anti-rabbit IgG(H + L) A21206, Alexa Fluor™ 594 goat anti-rabbit IgG(H + L) A11012, all from Invitrogen) each in a dilution of 1:1000 were applied respectively for 1 h at room temperature and DAPI (4’,6-diamidino-2-phenylindole) was used for nuclear staining. Primary antibodies were used at the following concentrations in 1% BSA/PBS upon application: mouse anti hTUBB3 (1:250), rabbit anti hMAP-2 (1:100), anti hIL-17RA (1:100), anti hIL-10RB (1:100), anti hTNFR1 (1:100), anti hIFNGR1 (1:100).

Meyer-Arndt L., Kerkering J., Kuehl T., Infante A.G., Paul F., Rosiewicz K.S., Siffrin V, & Alisch M. (2023). Inflammatory Cytokines Associated with Multiple Sclerosis Directly Induce Alterations of Neuronal Cytoarchitecture in Human Neurons. Journal of Neuroimmune Pharmacology, 18(1-2), 145-159.

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Comprehensive Antibody Panel for Cellular Analysis

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Antibodies against ALR (11293-1-AP, diluted 1:500), USP36 (14783-1-AP, diluted 1:500), MDM2 (27883-1-AP, diluted 1:500), fatty acid synthase (FASN, 10624-2-AP, diluted 1:500), Ki67 (27309-1-AP, diluted 1:2000), F4/80 (28463-1-AP, diluted 1:2000), smooth muscle actin (SMA, 14395-1-AP, diluted 1:1000), Mitofusin 2 (Mfn2, 12186-1-AP, diluted 1:1000), CD34 (14486-1-AP, diluted 1:1000), COX IV (11242-1-AP, diluted 1:1000), glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 60004-1-Ig, diluted 1:1000), goat anti-rabbit IgG-HRP (SA00001-2, diluted 1:5000), and goat anti-mouse IgG HRP (SA00001-1, diluted 1:5000) were purchased from Proteintech (Chicago, IL, USA). Antibodies targeting Drp1 (ab219596, diluted 1:1000) were obtained from Abcam (Cambridge, UK). Antibodies against phospho-Drp1 Ser616 (3455, diluted 1:500) and phospho-Drp1 Ser637 (4867, diluted 1:1000) were acquired from CST (Danvers, MA, USA) and those targeting MDM2 (sc-56154, diluted 1:500) and ubiquitin (sc-8017, diluted 1:500) from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against Flag (PM020, diluted 1:1000), Myc (M192-3, diluted 1:1000) and HA (PM020, diluted 1:1000) were purchased from MBL (Nagoya, Japan). Alexa Fluor 488 goat anti-mouse IgG (H+L) (A-11001, diluted 1:200) and Alexa Fluor 594 goat anti-rabbit IgG (H+L) (A-11012, diluted 1:200) were from Invitrogen (Carlsbad, CA, USA).

Zheng M., Ai Z., Guo Y., Chen Y., Xie P, & An W. (2023). Imbalance in ALR ubiquitination accelerates the progression of nonalcoholic steatohepatitis to hepatocellular carcinoma. Oncogene, 42(4).

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