0.01–0.03 g of harvested mycelia was homogenized in 1 ml of peqGOLD TriFast DNA/RNA/protein purification system reagent (VWR, part of Avantor Performance Materials, LLC, Radnor, PA, USA) using a FastPrep FP120 BIO101 ThermoSavant cell disrupter (Qbiogene, Carlsbad, US). RNA was isolated according to the manufacturer’s instructions, and the concentration was measured using the NanoDrop ONE (Thermo Scientific).
Fastprep fp120 bio101 thermosavant cell disrupter
Manufactured by Qbiogene
Sourced in Germany, United States
The FastPrep FP120 BIO101 ThermoSavant cell disrupter is a laboratory instrument designed for efficient and rapid disruption of biological samples, such as plant, animal, or microbial cells. Its core function is to break down cell walls and membranes to release intracellular contents, enabling further analysis or extraction of biomolecules.
Automatically generated - may contain errors
Lab products found in correlation
Peqgold trifast dna rna protein purification system reagent, by Avantor (5 mentions)
Nanodrop one, by Thermo Fisher Scientific (4 mentions)
Nanodrop 1000, by Thermo Fisher Scientific (4 mentions)
Revertaid h minus first strand cdna synthesis kit, by Thermo Fisher Scientific (4 mentions)
Mastercycler ep realplex 2.2 system, by Eppendorf (2 mentions)
Iq sybr green mix, by Bio-Rad (2 mentions)
Nanodrop 1000 spectrophotometer, by Thermo Fisher Scientific (2 mentions)
Revertaidtm h minus first strand cdna synthesis kit, by Thermo Fisher Scientific (1 mentions)
5 protocols using fastprep fp120 bio101 thermosavant cell disrupter
1
Mycelia RNA Extraction Using TriFast
2
Fungal Mycelia RNA Extraction and cDNA Synthesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Approximately 20 mg of harvested mycelia were homogenized in 1 ml of peqGOLD TriFast DNA/RNA/protein purification system reagent (PEQLAB Biotechnologie, Erlangen, Germany) using a FastPrep FP120 BIO101 ThermoSavant cell disrupter (Qbiogene, Carlsbad, CA, United States). RNA was isolated according to the manufacturer’s instructions, and the concentration was measured using the NanoDrop 1000 (Thermo Scientific). Synthesis of cDNA from mRNA was carried out using the RevertAidTM H Minus First Strand cDNA Synthesis Kit (Thermo Scientific) according to the manufacturer’s instructions.
Reverse transcription polymerase chain reactions (RT-PCRs) were performed in a Mastercycler® ep realplex 2.2 system (Eppendorf, Hamburg, Germany). All reactions were performed in triplicates. The amplification mixture (final volume 25 μl) contained 12.5 μl 2 × iQ SYBR Green Mix (Bio-Rad), 100 nM forward and reverse primer, and 2.5 μl cDNA (diluted 1:100) as template. All used primers are listed in Supplementary TableS1 . Cycling conditions and control reactions were performed as described earlier (Steiger et al., 2010 (link)).
Reverse transcription polymerase chain reactions (RT-PCRs) were performed in a Mastercycler® ep realplex 2.2 system (Eppendorf, Hamburg, Germany). All reactions were performed in triplicates. The amplification mixture (final volume 25 μl) contained 12.5 μl 2 × iQ SYBR Green Mix (Bio-Rad), 100 nM forward and reverse primer, and 2.5 μl cDNA (diluted 1:100) as template. All used primers are listed in Supplementary Table
3
Fungal RNA Extraction and cDNA Synthesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
0.01–0.03 g of harvested mycelia were homogenized in 1 mL of peqGOLD TriFast DNA/RNA/protein purification system reagent (PEQLAB Biotechnologie, Erlangen, Germany) using a FastPrep FP120 BIO101 ThermoSavant cell disrupter (Qbiogene, Carlsbad, USA). RNA was isolated according to the manufacturer’s instructions, and the concentration was measured using the NanoDrop 1000 (Thermo Scientific).
Synthesis of cDNA from mRNA was carried out using the RevertAid™ H Minus First Strand cDNA Synthesis Kit (Thermo Scientific) according to the manufacturer’s instructions.
Synthesis of cDNA from mRNA was carried out using the RevertAid™ H Minus First Strand cDNA Synthesis Kit (Thermo Scientific) according to the manufacturer’s instructions.
4
Fungal Mycelial RNA Extraction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
0.01–0.03 g of harvested mycelia were homogenized in 1 mL of peqGOLD TriFast DNA/RNA/protein purification system reagent (VWR, part of Avantor Performance Materials, LLC, Radnor, PA, USA) using a FastPrep FP120 BIO101 ThermoSavant cell disrupter (Qbiogene, Carlsbad, US). RNA was isolated according to the manufacturer’s instructions, and the concentration was measured using the NanoDrop ONE (Thermo Scientific).
5
Mycelia RNA Extraction and qPCR Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Approximately 20 mg of harvested mycelia was homogenized in 1 ml of peqGOLD TriFast DNA/RNA/protein purification system reagent (Peqlab Biotechnologie, Erlangen, Germany) using a FastPrep FP120 BIO101 ThermoSavant cell disrupter (Qbiogene, Carlsbad, CA). RNA was isolated according to the manufacturer's instructions, and the concentration was measured using a NanoDrop 1000 spectrophotometer (Thermo Scientific). Synthesis of cDNA from mRNA was carried out using the RevertAid H Minus first-strand cDNA synthesis kit (Thermo Scientific) according to the manufacturer's instructions.
Quantitative PCRs were performed in a Mastercycler ep realplex 2.2 system (Eppendorf, Hamburg, Germany). All reactions were performed in triplicates. The amplification mixture (final volume, 25 μl) contained 12.5 μl of 2× iQ SYBR green mix (Bio-Rad), 100 nM concentrations of the forward and reverse primers, and 2.5 μl of cDNA (diluted 1:100) as the template. All of the primers used are listed inTable 1 . Cycling conditions and control reactions were as described previously (23 (link)). Calculations using sar1 and act1 as reference genes were performed as published previously (23 (link)).
Quantitative PCRs were performed in a Mastercycler ep realplex 2.2 system (Eppendorf, Hamburg, Germany). All reactions were performed in triplicates. The amplification mixture (final volume, 25 μl) contained 12.5 μl of 2× iQ SYBR green mix (Bio-Rad), 100 nM concentrations of the forward and reverse primers, and 2.5 μl of cDNA (diluted 1:100) as the template. All of the primers used are listed in
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!