Single-cell suspensions were stained in FACS buffer at 4°C for 30 min. Intracellular cytokine to detect Th subsets was measured as previously described (Lim et al., 2014 (link)). Samples were analyzed using an LSRII (BD Pharmingen; USA). The following antibodies against mouse antigens were purchased from BD Pharmingen (USA): FITC-conjugated anti-H-2d, anti-IL-17A, and anti-IL-4; and PE-conjugated anti-CD8; and PerCP-Cy5.5-conjugated anti-CD62L and anti-CD8; and APC-conjugated anti-CD44, anti-IFNγ, anti-Foxp3, Annexin V; and APC-Cy7-conjugated anti-CD4. Alexa Fluor 700-conjugated anti-CD45.1 was purchased from ebioscience (USA).
Percp cy5.5 conjugated anti cd62l
Manufactured by BD
Sourced in United States
PerCP-Cy5.5-conjugated anti-CD62L is a fluorescently-labeled monoclonal antibody that binds to the CD62L cell surface antigen. CD62L, also known as L-selectin, is a cell adhesion molecule involved in lymphocyte homing and trafficking. The PerCP-Cy5.5 fluorescent label allows for the detection and analysis of CD62L-expressing cells using flow cytometry.
Automatically generated - may contain errors
2 protocols using percp cy5.5 conjugated anti cd62l
1
Multiparametric Flow Cytometry Analysis
2
ZIKV E Antigen-Stimulated Splenocyte Response
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A total of 5×105 splenocytes (treated as in the splenocyte proliferation assay) along with purified ZIKV E antigen (10 μg/ml) were plated in 96-well plates. After culture for 72 h, the cells were collected in PBS containing 2% FBS and 0.1% NaN3. Then, the cells were stained with 0.25 μg of FITC-conjugated anti-CD4, PE-conjugated anti-CD8, APC-conjugated anti-CD19, PE/Cy7-conjugated anti-CD69, APC-conjugated anti-CD44 or PerCP-Cy5.5-conjugated anti-CD62L (BD Biosciences, Franklin, CA, USA) antibodies for 30 min at 4°C. Data were acquired on a FACSCalibur flow cytometer (BD Biosciences, Franklin, CA, USA).
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