Immunohistochemistry was performed on paraffin-embedded tissues sections (3-4 μm-thick) using the following primary antibodies: ER-α (clone 6F11, monoclonal; Novocastra, Newcastle-Upon-Tyne, UK), PgR (clone PgR636, monoclonal; DakoCytomation, Glostrup, Denmark) and HER2 (A0485, polyclonal; DakoCytomation). Diaminobenzidine (Dako) was used as the chromogen. ER and PgR expression was considered positive when at least 10% of invasive tumoral cells exhibited nuclear staining, regardless of intensity. For HER2, the immunohistochemical score was assigned according to the Herceptest scoring system [19 (link)].
Pgr clone pgr636
Manufactured by Agilent Technologies
Sourced in United Kingdom
The PgR (clone PgR636) is a laboratory equipment product offered by Agilent Technologies. It is designed to detect and measure the expression of progesterone receptor (PgR) in biological samples. The core function of this product is to provide researchers with a reliable tool for analyzing PgR levels in their experiments.
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Lab products found in correlation
2 protocols using pgr clone pgr636
1
Immunohistochemical Biomarker Evaluation
2
Immunohistochemical Analysis of Breast Cancer
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All patients underwent a core needle biopsy prior to NAC and curative surgery involving mastectomy or conservative surgery with axillary lymph node dissection after NAC. Immunohistochemical studies were performed as described previously.17 (link) Primary monoclonal antibodies directed against ER (clone 1D5, dilution 1:80; Dako), PgR (clone PgR636, dilution 1:100; Dako), HER2 (HercepTest; Dako), Ki-67 (clone MIB-1, dilution 1:00; Dako), CD8 (clone C8/144B, dilution 1:100; Dako) and FOXP3 (clone 236A/E7, dilution 1:100; Abcam, Cambridge, UK) were used.
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