Embryomax 0.1 gelatin solution

Manufactured by Merck Group

Sourced in Germany, United Kingdom

EmbryoMax® 0.1% Gelatin Solution is a sterile, cell culture-tested solution containing 0.1% gelatin in Dulbecco's Phosphate Buffered Saline (DPBS). It is designed for use as a cell culture substrate.

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4 protocols using embryomax 0.1 gelatin solution

Extracellular Action Potential Recordings of NRVCs

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NRVCs were plated on MEA plates (Multichannel Systems, Germany) pre-coated with EmbryoMax® 0.1% Gelatin Solution (Millipore) at a density of 1000 cells/mm². Each MEA plate contains 60 titanium nitrate (TiN) electrodes with diameter of 30 μM positioned in a rectangular grid. Extracellular action potential was recorded at initial state before any transduction using MEA-1060 system (Multichannel Systems, Germany). Cells were then transduced with either Ad-Tat or Ad-null and extracellular action potential was recorded post transduction. The sampling frequency was set to 2000 kHz and an online built-in bandpass filter was utilized throughout the recording. Data were recorded using the MC_Rack software and further analyzed with MATLAB®.

Tahrir F.G., Shanmughapriya S., Ahooyi T.M., Knezevic T., Gupta M.K., Kontos C.D., McClung J.M., Madesh M., Gordon J., Feldman A.M., Cheung J.Y, & Khalili K. (2017). Dysregulation of Mitochondrial Bioenergetics and Quality Control by HIV-1 Tat in Cardiomyocytes. Journal of cellular physiology, 233(2), 748-758.

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Macrophage Differentiation and Characterization

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RPMI medium, DPBS without Ca²⁺ and Mg²⁺, penicillin-streptomycin, Glutamax, microplate BCA protein assay, trypsin-EDTA, and TEAB were from Thermo Scientific, Waltham, MA, USA; trypsin protease-MS grade, sodium dodecyl sulphate, acetonitrile, HPLC-grade ethanol, and water were from Sigma, Dorset, UK; human Fc block and Alexa Fluor^® anti-human CD300A Cat# 566342 were from BD Bioscience, Berkshire, UK; PE anti-human CD68 Cat# 130-118-486 was from Miltenyi Biotec, Surrey, UK; PE anti-human CD109 Cat# 323305, APC anti-human LILRB2 Cat# 338707, APC anti-human Siglec-10 Cat# 347606, APC anti-human CD206 Cat# 321109, APC anti-human CD80 Cat# 305219, APC anti-human CD86 Cat# 374208, PE/Cy7 anti-human CD14 Cat# 367112, and recombinant human M-CSF were from Biolegend, San Diego, CA, USA. X-VIVO15 Serum free medium Cat# BE02-060Q was from Lonza, Basel, Switzerland. Recombinant human BMP4 (Cat# 120-05), SCF (Cat# 300-07), VEGF (Cat# 100-20) and IL-3 (Cat# 200-03) were from Peprotech, London, UK. EmbryoMax^® 0.1% Gelatin solution was from Millipore, London, UK. Y-27632 (Cat# 1254/10) was from Bio-Techne, Abingdon, UK.

Murugesan G., Davidson L., Jannetti L., Crocker P.R, & Weigle B. (2022). Quantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells. Biomedicines, 10(2), 239.

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Feeder-free Maintenance of Undifferentiated ESCs

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Undifferentiated ESCs were maintained feeder free on gelatin (EmbryoMax 0.1% gelatin solution, ES-006-B; Millipore)-coated plates, in knockout DMEM (10829-018; Gibco), 20% knockout serum replacement (KSR, 10828-028; Gibco), 2 mM L-glutamine (25030-024; Gibco), 1x minimal essential medium nonessential amino acids (11140-035; Gibco), 1× penicillin-streptomycin (15140-122; Gibco), 100 μM β-mercaptoethanol (31350; Gibco) and 1,000 U/mL recombinant LIF (ESG1107; Chemicon International).

Lo Nigro A., de Jaime-Soguero A., Khoueiry R., Cho D.S., Ferlazzo G.M., Perini I., Abon Escalona V., Aranguren X.L., Chuva de Sousa Lopes S.M., Koh K.P., Conaldi P.G., Hu W.S., Zwijsen A., Lluis F, & Verfaillie C.M. (2017). PDGFRα+ Cells in Embryonic Stem Cell Cultures Represent the In Vitro Equivalent of the Pre-implantation Primitive Endoderm Precursors. Stem Cell Reports, 8(2), 318-333.

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Maintenance and Wnt Activation of Mouse ESCs

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R1 and E14Tg2 mouse ESCs were maintained feeder-free on gelatin- (EmbryoMax 0.1% Gelatin Solution, ES-006-B; Millipore) coated plates in DMEM (41965–039 Gibco), 15% fetal bovine serum (Sigma), 2 mM L-glutamine (25030–024; Gibco), 1X minimal essential medium non-essential amino acids (Gibco), penicillin (100 U/ml) /streptomycin (100U/ml) (15140122; Gibco), 100 μM β-Mercaptoethanol (Gibco) and 1,000 U/ml recombinant mouse leukemia inhibitory factor (ESG1107; ESGRO, Chemicon International). mESCs were treated at indicated concentrations to activate the Wnt pathway: purified Wnt3a (315–20; Peprotech); BIO (361550; Calbiochem); CHIR99021 (361571; Calbiochem). BIO and CHIR99021 were resuspended in DMSO (Sigma) at a stock concentration of 2 mM (BIO) and 6 or 10 mM (CHIR99021), Wnt3a (Peprotech) was resuspended at a stock concentration of 50ng/μL following manufacturer instructions.

De Jaime-Soguero A., Aulicino F., Ertaylan G., Griego A., Cerrato A., Tallam A., del Sol A., Cosma M.P, & Lluis F. (2017). Wnt/Tcf1 pathway restricts embryonic stem cell cycle through activation of the Ink4/Arf locus. PLoS Genetics, 13(3), e1006682.

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