Krt8 creer

Mice were housed in an AAALAC accredited animal facility at the University of Colorado Boulder under barrier laboratory conditions in a temperature- and relative humidity-controlled (i.e., 20 ± 1 C and 40%, respectively) environment with a normal 12-h light-dark cycle. Mice were housed in sterile, static filter-top shoebox caging, with sanichip bedding, nestlet enrichment, irradiated food and R/O water available ad libitum.
The following mouse lines were used: Np63-GFP (Romano et al., 2012 (link)), Ctnnb1 fl/fl (Jackson Lab, #004152), Krt8-CreER (Jackson Lab, #017947), Pdgfra-CreER (Jackson Lab, #018280), BAT-GAL (Jackson Lab, #005317), Np63-Cre (Jackson Lab, #024564) and Rosa26-LSL-tdTomato (Jackson Lab, #021876).
Krt8-CreER and Pdgfra-CreER induction was performed in pregnant female animals at embryonic day 9 (E9) and analyzed at E13.Tamoxifen dissolved in 10% Ethonal and 90% peanut oil (20mg/ml) was injected into pregnant females (4mg/animal) by intraperitoneal (IP) injection. All animal experiments were approved by the IACUC at the University of Colorado Boulder. For all experiments that used mouse embryos, male and female embryos were used indistinguishably because no differences were detected at the phenotypical level between male and female embryos.

All animal husbandry and procedures were performed in compliance with institutional animal care and use committee guidelines. Crhr2-ires-Cre and Gabra1-ires-Cre mice were constructed as described below; Gpr65-ires-Cre, Glp1r-ires-Cre, Npy2r-ires-Cre, P2ry1-ires-Cre and P2×2/P2×3^−/− mice were described before (Chang et al., 2015 (link); Finger et al., 2005 ; Williams et al., 2016 (link)); Vglut2-ires-Cre and loxP-L10-GFP mice were gifts from Bradford Lowell (Beth Israel Deaconess Medical Center); and wild type C57BL/6J (000664), Calb1-ires-Cre (028532), Chat-ires-Cre (006410), Chat-GFP (007902), Krt8-Cre^ER (017947), Npy1r-Cre (030544), Piezo2-EGfp-ires-Cre (027719), Plcβ2^−/− (018064), loxP-tdTomato (007908), loxP-ChR2 (012569), and loxP-DTR (007900) mice were purchased (Jackson Laboratory). The constitutive GCaMP3 allele (Rosa26-GCaMP3) was generated by breeding loxP-GCaMP3 (014538) with E2a-Cre mice (Jackson, 003314), and then crossing out the E2a-Cre allele. Single-cell sequencing was performed on male C57BL/6J mice; both male and female mice between 8–24 weeks old were used for all other studies, and no differences based on sex were observed.