Plasmid pRP-Puro-CMV-CC16 and its vector pRP-Puro-CMV were purchased from VectorBuilder (Chicago, IL). Human HSP60 cDNA amplified by RT-PCR was subcloned into the Kpn I and Xho I sites of pcDNA3.1 vector (CMV promoter; Invitrogen) to obtain pcDNA-HSP60, and the primer sequences used for cloning are provided in Table S3 . pEGFP-C1-PKM2 (Addgene plasmid #64698) was a gift from Axel Ullrich.73 (link)
Prp puro cmv
Manufactured by VectorBuilder
Sourced in United States
The PRP-Puro-CMV is a lentiviral vector that expresses the puromycin resistance gene under the control of the cytomegalovirus (CMV) promoter. It is designed for the selection of cells that have been transduced with the lentiviral vector.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using prp puro cmv
1
Plasmid Construction for Gene Expression
2
Generating Stable Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plasmids pRP-Puro-CMV and pRP-Puro-CMV-CC16 were purchased from VectorBuilder Inc. (Chicago, IL, USA). In order to generate stable cell lines, plasmids were transfected into BEAS-2B. Two days post-transfection, 100 µg/mL of hygromycin B was added to the culture medium in order to select plasmid-containing cells. The hygromycin-added media were changed every 3 days until colonies formed.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!