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Ls c109221

Manufactured by Thermo Fisher Scientific
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LS-C109221 is a laboratory equipment product offered by Thermo Fisher Scientific. It is a precision instrument designed for specialized applications in the scientific and research community.

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Lab products found in correlation

Cd31 pe cy7, by Thermo Fisher Scientific (2 mentions) Clone 30 f11, by Thermo Fisher Scientific (2 mentions) Epcam apc, by Thermo Fisher Scientific (2 mentions) Cd45 pe cy7, by Thermo Fisher Scientific (2 mentions) Clone 390, by Thermo Fisher Scientific (2 mentions) Clone g8, by Thermo Fisher Scientific (2 mentions) Pdpn efluor660, by Thermo Fisher Scientific (2 mentions) Epcam efluor488, by Thermo Fisher Scientific (2 mentions) Sheep anti mouse tm4sf1, by R&D Systems (2 mentions) Af7514, by R&D Systems (2 mentions) Anti sheep 488 secondary, by Abcam (2 mentions)

2 protocols using ls c109221

1

Isolation and Characterization of Lung Stem and Progenitor Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Lungs from Axin2CreERT2-TdTomato animals were harvested at 6–8 weeks of age and processed into a single cell suspension using dispase, collagenase I, and DNase as previously described36 (link),37 (link). EPCAM+ Axin2+ cells (TdTomato+) were identified via FACS sorting as previously described37 (link). The total AT2 population (Sftpc+ AT2s) was isolated from lungs of 6–8 week old SftpcCreERT2:R26REYFP animals 5 days after induction with 200μg/gm tamoxifen. EYFP+ cells were then isolated via FACS sorting as previously described37 (link). For sorting and quantification, the following antibodies were used: Pdpn-eFluor660 (eBioscience, Clone 8.1.1, 1:100) EpCAM-APC (eBioscience, Clone G8.8, 1:200), EpCAM-eFluor488 (eBioscience, Clone G8.8, 1:200), CD31-PeCy7 (eBioscience, Clone 390, 1:200), CD45-PeCy7 (eBioscience, Clone 30-F11, 1:200). Two anti-mouse Tm4sf1 antibodies were used to ensure specificity: Sheep anti-mouse Tm4sf1 (R&D systems, AF7514, 1:10) and Sheep IgG isotype control (R&D systems, 5-001-A, 1:10) with anti-Sheep 488 secondary (Abcam, ab150177, 1:50) or Rabbit anti-mouse Tm4sf1 (LS Biosciences, B7077, 1:25) and Rabbit IgG isotype control (LS Biosciences, LS-C109221, 1:25) with Donkey anti-rabbit 488 secondary (Life Technologies, A212016, 1:200).
Zacharias W.J., Frank D.B., Zepp J.A., Morley M.P., Alkhaleel F., Kong J., Zhou S., Cantu E, & Morrisey E.E. (2018). Regeneration of the lung alveolus by an evolutionarily conserved epithelial progenitor. Nature, 555(7695), 251-255.
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2

Isolation and Characterization of Lung Stem and Progenitor Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Lungs from Axin2CreERT2-TdTomato animals were harvested at 6–8 weeks of age and processed into a single cell suspension using dispase, collagenase I, and DNase as previously described36 (link),37 (link). EPCAM+ Axin2+ cells (TdTomato+) were identified via FACS sorting as previously described37 (link). The total AT2 population (Sftpc+ AT2s) was isolated from lungs of 6–8 week old SftpcCreERT2:R26REYFP animals 5 days after induction with 200μg/gm tamoxifen. EYFP+ cells were then isolated via FACS sorting as previously described37 (link). For sorting and quantification, the following antibodies were used: Pdpn-eFluor660 (eBioscience, Clone 8.1.1, 1:100) EpCAM-APC (eBioscience, Clone G8.8, 1:200), EpCAM-eFluor488 (eBioscience, Clone G8.8, 1:200), CD31-PeCy7 (eBioscience, Clone 390, 1:200), CD45-PeCy7 (eBioscience, Clone 30-F11, 1:200). Two anti-mouse Tm4sf1 antibodies were used to ensure specificity: Sheep anti-mouse Tm4sf1 (R&D systems, AF7514, 1:10) and Sheep IgG isotype control (R&D systems, 5-001-A, 1:10) with anti-Sheep 488 secondary (Abcam, ab150177, 1:50) or Rabbit anti-mouse Tm4sf1 (LS Biosciences, B7077, 1:25) and Rabbit IgG isotype control (LS Biosciences, LS-C109221, 1:25) with Donkey anti-rabbit 488 secondary (Life Technologies, A212016, 1:200).
Zacharias W.J., Frank D.B., Zepp J.A., Morley M.P., Alkhaleel F., Kong J., Zhou S., Cantu E, & Morrisey E.E. (2018). Regeneration of the lung alveolus by an evolutionarily conserved epithelial progenitor. Nature, 555(7695), 251-255.
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