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Isolectin gs ib4 alexa 647

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Isolectin GS-IB4-Alexa 647 is a fluorescently labeled lectin used for the detection and localization of glycoconjugates in biological samples. It is a protein derived from the seeds of the plant Griffonia simplicifolia that binds specifically to alpha-D-galactose and N-acetyl-alpha-D-galactosamine residues. The Alexa Fluor 647 dye is covalently attached to the lectin, allowing for visualization of the bound glycoconjugates using fluorescence microscopy or flow cytometry.

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Lab products found in correlation

Anti tomm20, by Abcam (2 mentions) Isolectin gs ib4 alexa 488, by Thermo Fisher Scientific (2 mentions) Isolectin gs ib4 alexa 568, by Thermo Fisher Scientific (2 mentions) Anti ng2 chondroitin sulfate proteoglycan, by Merck Group (2 mentions) Anti collagen 4, by Southern Biotech (2 mentions) Anti cpt1a, by Cell Signaling Technology (2 mentions)

2 protocols using isolectin gs ib4 alexa 647

1

Immunofluorescence Staining of Fixed Cells

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PFA fixed cell cultures (4% PFA except for CPT1a staining where 2% PFA was used), spheroids (4% PFA) or whole mount retinas (4% PFA) were subjected to immunofluorescence staining using the following isolectin conjugates or primary antibodies: isolectin GS-IB4-Alexa 488, isolectin GS-IB4-Alexa 568, isolectin GS-IB4-Alexa 647 (Molecular Probes), anti-CPT1a (Cell Signaling), anti-NG2 Chondroitin Sulfate Proteoglycan (Chemicon), anti-Tomm20 (Abcam) and anti-collagen IV (Southern Biotech). Alexa-488, -568 or -633 conjugated secondary antibodies were used (Molecular Probes). EdU, EU and HPG staining was performed using a Click-IT assay with Alexa fluor 555 according to manufacturer’s instructions.
Schoors S., Bruning U., Missiaen R., Queiroz K.C., Borgers G., Elia I., Zecchin A., Cantelmo A.R., Christen S., Goveia J., Heggermont W., Goddé L., Vinckier S., Van Veldhoven P.P., Eelen G., Schoonjans L., Gerhardt H., Dewerchin M., Baes M., De Bock K., Ghesquière B., Lunt S.Y., Fendt S.M, & Carmeliet P. (2015). Fatty acid carbon is essential for dNTP synthesis in endothelial cells. Nature, 520(7546), 192-197.
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2

Immunofluorescence Staining of Fixed Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
PFA fixed cell cultures (4% PFA except for CPT1a staining where 2% PFA was used), spheroids (4% PFA) or whole mount retinas (4% PFA) were subjected to immunofluorescence staining using the following isolectin conjugates or primary antibodies: isolectin GS-IB4-Alexa 488, isolectin GS-IB4-Alexa 568, isolectin GS-IB4-Alexa 647 (Molecular Probes), anti-CPT1a (Cell Signaling), anti-NG2 Chondroitin Sulfate Proteoglycan (Chemicon), anti-Tomm20 (Abcam) and anti-collagen IV (Southern Biotech). Alexa-488, -568 or -633 conjugated secondary antibodies were used (Molecular Probes). EdU, EU and HPG staining was performed using a Click-IT assay with Alexa fluor 555 according to manufacturer’s instructions.
Schoors S., Bruning U., Missiaen R., Queiroz K.C., Borgers G., Elia I., Zecchin A., Cantelmo A.R., Christen S., Goveia J., Heggermont W., Goddé L., Vinckier S., Van Veldhoven P.P., Eelen G., Schoonjans L., Gerhardt H., Dewerchin M., Baes M., De Bock K., Ghesquière B., Lunt S.Y., Fendt S.M, & Carmeliet P. (2015). Fatty acid carbon is essential for dNTP synthesis in endothelial cells. Nature, 520(7546), 192-197.
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