Micromassqtof 2 mass spectrometer

Manufactured by Waters Corporation

The MicromassQTOF-2 mass spectrometer is a high-performance analytical instrument designed for accurate mass determination and structural elucidation of chemical compounds. It utilizes a quadrupole time-of-flight (QTOF) configuration to provide precise mass measurements and detailed structural information.

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Lab products found in correlation

Drx 700 spectrometer, by Bruker (1 mentions) Uv 265 spectrophotometer, by Shimadzu (1 mentions) Infrared 400 spectrophotometer, by Shimadzu (1 mentions) Silica gel 60, by Merck Group (1 mentions) Silica gel, by Merck Group (1 mentions) Cary 300 bio uv vis spectrophotometer, by Agilent Technologies (1 mentions) Emxplus spectrometer, by Bruker (1 mentions) Unity inova 400 mhz spectrometer, by Agilent Technologies (1 mentions) Icap 6300, by Thermo Fisher Scientific (1 mentions)

3 protocols using micromassqtof 2 mass spectrometer

Characterization of Marinobactin and Aquachelin

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Electrospray ionization mass
spectrometry (ESI-MS) and tandem mass spectrometry using a Micromass
QTOF-2 mass spectrometer (Waters Corp.) in positive ion mode with
argon as a collision gas were used to determine the mass and partial
amino acid connectivity of the marinobactin and aquachelin head groups.
For M_HG, ¹H, ¹³C, and various two-dimensional
nuclear magnetic resonance (NMR) techniques, including ¹H–¹H correlation spectroscopy (COSY), heteronuclear
multiple quantum coherence (HMQC), and heteronuclear multiple bond
correlation (HMBC), were recorded on a 800 MHz JEOL DELTA2 ECA800
spectrometer. A ¹H–¹⁵N heteronuclear
single quantum coherence (¹⁵N-HSQC) NMR spectrum of the ¹⁵N-incorporated M_HG was recorded on a 500 MHz JEOL
DELTA2 ECA500 instrument. M_HG was dissolved in dimethyl
sulfoxide-d₆ (DMSO-d₆, 99.9%, Cambridge Isotope, Inc.) (d = 2.46
ppm), and experiments were run at 23.5 °C at the National Institute
of Health Sciences in Tokyo, Japan.

Gauglitz J.M., Iinishi A., Ito Y, & Butler A. (2014). Microbial Tailoring of Acyl Peptidic Siderophores. Biochemistry, 53(16), 2624-2631.

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Structural Characterization of Compounds

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Melting points were measured on a Buchi melting point B‐545 apparatus (Boston Laboratory Equipment) and not corrected. UV spectrum was recorded on a Shimadzu UV‐265 spectrophotometer, in MeOH, and IR spectrum on a Shimadzu Infrared 400 spectrophotometer, in KBr pellets. ¹H‐NMR ¹³C‐NMR and 2D‐NMR spectra were determined by a Bruker DRX 700 spectrometer, in CDCl₃ and DMSO‐d₆. Chemical shifts were measured in d values (ppm) with tetramethylsilane (TMS) as an internal reference. HRESIMS was measured in a MICROMASS Q‐Tof 2 mass spectrometer, Waters Corporation. Vacuum liquid chromatography (VLC) was performed using silica gel 60 (0.04–0.063 mm; 500 g; Merck). Column chromatography (CC): silica gel (Merck, 60–120 mesh) TLC zones were visualized either by exposure to vanillin sulfuric acid, iodine vapor, or under UV light. All evaporations were achieved in a vacuum on a rotary evaporator.

Al‐Salem H.S., Al‐Yousef H.M., Ashour A.E., Ahmed A.F., Amina M., Issa I.S, & Bhat R.S. (2020). Antioxidant and hepatorenal protective effects of bee pollen fractions against propionic acid‐induced autistic feature in rats. Food Science & Nutrition, 8(9), 5114-5127.

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Spectroscopic Analysis of Chemical Samples

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All UV-visible (UV-Vis) spectrophotometry was carried out on a Varian Cary-Bio 300 UV-Vis spectrophotometer. 1 H NMR spectra were recorded on a Varian Unity Inova 400 MHz spectrometer (5 mm broad band probe) at room temperature. Mass spectra were obtained using electrospray ionization mass spectrometry (ESI-MS) and tandem mass spectrometry (ESI-MS/MS) on a Micro Mass QTOF-2 mass spectrometer (Waters Corp.), using argon as the collision gas. Inductively coupled plasma atomic emission spectroscopy (ICP-AES), measurements were taken on a Thermo iCAP 6300, and calibrated from dilutions of 1000 ppm Mn(II) or Fe(III) standard solutions (Fisher), using class A volumetric glassware. X-Band EPR spectra were obtained using a Bruker EMXplus Spectrometer with an aqueous flat cell. Prior to inoculation, 20 mL of filter-sterilized 1.0 M HEPES buffer (pH 7.4), and 4 mL of filtersterilized 1.0 M NaHCO 3 buffer were added to the medium.

Springer S.D, & Butler A. (2015). Magnetic susceptibility of Mn(III) complexes of hydroxamate siderophores. Journal of inorganic biochemistry, 148.

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