Western-blotting was used to detect and analyze the levels of proteins in cells and tumor tissues. Total proteins were extracted from cells or tissues, quantified with the BCA reagent method, and then separated by 8% or 10% (w/v) SDS-PAGE. The protein was transferred to a PVDF membrane (Bio-Rad, Hercules), blocked for 1.5 h with 5% (w/v) skim milk in TBST at room temperature, and incubated overnight at 4 °C with the Prdx1 antibody (1:10000, Abcam), NEDD9 antibody (1:1000, Cell signaling technology), p-Aurora A antibody (1:1000, Gentex), Aurora A antibody (1:10000, Abcam), HDAC6 antibody (1:10000, Abcam), or the GAPDH antibody (1:10000, Abcam). The membranes were washed three times with TBST and then incubated with horseradish peroxidase-conjugated (HRP) secondary antibody for 1 h at room temperature. The protein signal was detected using a protein gel imaging system (ChemiDocXRS + Imaging System; Bio-Rad).
Hdac6 antibody
Manufactured by Abcam
HDAC6 antibody is a protein that binds to and inhibits the activity of the enzyme HDAC6, which is involved in the deacetylation of proteins. This antibody can be used for the detection and study of HDAC6 in various biological applications.
Automatically generated - may contain errors
Lab products found in correlation
Aurora a antibody, by Abcam (4 mentions)
Prdx1 antibody, by Abcam (4 mentions)
Nedd9 antibody, by Cell Signaling Technology (4 mentions)
P aurora a antibody, by Gentex (4 mentions)
Gapdh antibody, by Abcam (4 mentions)
Pvdf membrane, by Bio-Rad (4 mentions)
Chemidoc xrs imaging system, by Bio-Rad (4 mentions)
4 protocols using hdac6 antibody
1
Western Blot Analysis of Protein Levels
2
Protein Expression Analysis by Western Blotting
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Western-blotting was used to detect and analyze the levels of proteins in cells and tumor tissues. Total proteins were extracted from cells or tissues, quanti ed with the BCA reagent method, and then separated by 8% or 10% (w/v) SDS-PAGE. The protein was transferred to a PVDF membrane (Bio-Rad, Hercules), blocked for 1.5 h with 5% (w/v) skim milk in TBST at room temperature, and incubated overnight at 4 ℃ with the Prdx1 antibody (1:10000, Abcam), NEDD9 antibody (1:1000, Cell signaling technology), p-Aurora A antibody (1:1000, Gentex), Aurora A antibody (1:10000, Abcam), HDAC6 antibody (1:10000, Abcam), or the GAPDH antibody (1:10000, Abcam). The membranes were washed three times with TBST and then incubated with horseradish peroxidaseconjugated (HRP) secondary antibody for 1 h at room temperature. The protein signal was detected using a protein gel imaging system (ChemiDocXRS + Imaging System; Bio-Rad).
3
Quantitative Protein Analysis via Western Blotting
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Western-blotting was used to detect and analyze the levels of proteins in cells and tumor tissues. Total proteins were extracted from cells or tissues, quantified with the BCA reagent method, and then separated by 8% or 10% (w/v) SDS-PAGE. The protein was transferred to a PVDF membrane (Bio-Rad, Hercules), blocked for 1.5 h with 5% (w/v) skim milk in TBST at room temperature, and incubated overnight at 4 with the Prdx1 antibody (1:10000, Abcam), NEDD9 antibody (1:1000, Cell signaling technology), p-Aurora A antibody (1:1000, Gentex), Aurora A antibody (1:10000, Abcam), HDAC6 antibody (1:10000, Abcam), or the GAPDH antibody (1:10000, Abcam). The membranes were washed three times with TBST and then incubated with horseradish peroxidase-conjugated (HRP) secondary antibody for 1 h at room temperature. The protein signal was detected using a protein gel imaging system (ChemiDocXRS + Imaging System; Bio-Rad).
4
Protein Expression Analysis by Western Blotting
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Western-blotting was used to detect and analyze the levels of proteins in cells and tumor tissues. Total proteins were extracted from cells or tissues, quanti ed with the BCA reagent method, and then separated by 8% or 10% (w/v) SDS-PAGE. The protein was transferred to a PVDF membrane (Bio-Rad, Hercules), blocked for 1.5 h with 5% (w/v) skim milk in TBST at room temperature, and incubated overnight at 4 ℃ with the Prdx1 antibody (1:10000, Abcam), NEDD9 antibody (1:1000, Cell signaling technology), p-Aurora A antibody (1:1000, Gentex), Aurora A antibody (1:10000, Abcam), HDAC6 antibody (1:10000, Abcam), or the GAPDH antibody (1:10000, Abcam). The membranes were washed three times with TBST and then incubated with horseradish peroxidaseconjugated (HRP) secondary antibody for 1 h at room temperature. The protein signal was detected using a protein gel imaging system (ChemiDocXRS + Imaging System; Bio-Rad).
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