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Fructose 6 phosphate disodium salt

Manufactured by Thermo Fisher Scientific
Sourced in Germany

Fructose 6-phosphate disodium salt is a chemical compound used as a reagent in biochemical and analytical applications. It serves as a substrate for various enzymes involved in carbohydrate metabolism. The product is supplied as a crystalline powder.

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2 protocols using fructose 6 phosphate disodium salt

1

Yeast Enzyme Characterization Protocol

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Yeast enolase, monosodium phosphoenolpyruvate, and phosphoglucose isomerase type III from the baker’s yeast were purchased from Sigma-Aldrich (Sigma-Aldrich Chemie GmbH, Steinheim, Germany), fructose 6-phosphate disodium salt were from Alfa Aesar (Thermo Fisher (Kandel) GmbH, Kandel, Germany), MOPS was from AppliChem (AppliChem GmbH, Darmstadt, Germany), sodium chloride was from CHEMSOLUTE (Th. Geyer GmbH & Co. KG, Renningen, Germany), and magnesium chloride hexahydrate and sodium hydroxide were from Roth (Bernd Kraft, Duisburg, Germany). An overview about all the used chemicals, the CAS-numbers, and purity is given in Table S1 in the Supplementary Materials (SM).
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2

Enzymatic Assay for Yeast Enolase

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Yeast enolase, monosodium phosphoenolpyruvate, phosphoglucose isomerase type III from baker’s yeast and bovine serum albumin (BSA) were purchased from Sigma Aldrich (Sigma-Aldrich Chemie GmbH, Steinheim, Germany), fructose 6-phosphate disodium salt from Alfa Aesar (Thermo Fisher (Kandel) GmbH, Kandel, Germany), MOPS from AppliChem (AppliChem GmbH, Darmstadt, Germany), sodium chloride from CHEMSOLUTE (Th. Geyer GmbH & Co. KG, Renningen, Germany), polyethylene glycol with an average molar mass of 20,000 g mol−1 (PEG 20,000) from Merck (Merck KGaA, Darmstadt, Germany), polyethylene glycol 6000 from Serva (SERVA Electrophoresis GmbH, Heidelberg, Germany), magnesium chloride hexahydrate and sodium hydroxide from Roth (Bernd Kraft, Duisburg, Germany). An overview of all used chemicals, the CAS-numbers and purity is given in Table S1 in the supplementary materials.
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