Anti dr4

6-Gingerol was purchased from LKT Laboratories (St. Louis, MO, USA). Treatments of drugs were accomplished by aspirating the medium and replacing it with medium containing these drugs. Soluble human recombinant SuperKillerTRAIL (referred to as TRAIL in this manuscript) was purchased from Enzo Biochemicals (Enzo Life Sciences), diluted, and stored in KillerTRAIL storage and dilution buffer (Enzo Life Sciences). 6-Carboxy-2′,7′-dichlorofluorescein diacetate (H2DCF-DA) and dihydroethidium (DHE) were from Molecular Probe. N-acetylcysteine was from Sigma. Anti-c-FLIP, anti-Bax, anti-Bcl-2, and anti-p53 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-XIAP, anti-survivin, anti-Bid, anti-phospho ERK and anti-ERK, anti-phospho-p38, anti-p38, anti-cleaved caspase-3, anti-caspase-8, and anti-PARP-1 were purchased from Cell Signaling (Beverly, MA, USA). Anti-phospho JNK and anti-JNK were purchased from Upstate Biotechnology (Lake Placid, NY, USA). Anti-Flag M2 were purchased from Sigma (USA). Anti-DR4 and anti-DR5 were purchased from R&D Systems (Plymouth Metting, PA, USA). Anti-actin antibody was purchased from MP Biomedicals (Solon, OH, USA). For the secondary antibodies, anti-mouse-IgG-HRP and anti-rabbit-IgG-HRP were purchased from Santa Cruz Biotechnology.