Human melanoma cell lines (WM35, WM451, and A375) and HM were purchased from the ATCC (Rockville, MD, USA) and cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum (Gibco, Carlsbad, USA) and 1% penicillin/streptomycin under a humidified atmosphere of 5% CO2 at 37 °C. Human CMM tissues and corresponding normal skin tissues were collected from patients who underwent surgery and were pathologically diagnosed with MM at the Third Xiangya Hospital of the Central South University and Hunan Cancer Hospital from January 2015 to December 2017. Informed consent was obtained from all the participants.
Wm451
Manufactured by American Type Culture Collection
Sourced in United States
The WM451 is a laboratory equipment product designed for cell culture applications. It provides a consistent environment for the growth and maintenance of cells. The core function of the WM451 is to maintain temperature, humidity, and gas levels within optimal ranges to support cell viability and proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Sk mel 24, by American Type Culture Collection (2 mentions)
Hema lp, by American Type Culture Collection (1 mentions)
Anti mir 205, by GenePharma (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
4 protocols using wm451
1
Cultivating Human Melanoma Cell Lines
2
Melanoma cell culture and tissue collection
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Human malignant melanoma cell lines (WM35, WM451, and A375) and human melanocytes (HM) were purchased from the ATCC (Rockville, MD, USA) and cultured in Dulbecco's modi ed Eagle's medium (DMEM) supplemented with 10% foetal bovine serum (FBS; Gibco, Carlsbad, CA, USA) and 1% penicillin/streptomycin under a humidi ed atmosphere of 5% CO 2 at 37 °C. Human CMM tissues and corresponding adjacent non-cancerous skin tissues were collected from patients who underwent surgery and were pathologically diagnosed with MM at the Third Xiangya Hospital of the Central South University and Hunan Cancer Hospital from January 2015 to December 2017. Informed consent was obtained from all the participants.
3
Melanoma Cell Line Transfection Assay
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The human epidermal melanocytes (HEMa-LP) and melanoma cell lines (A375, SK-MEL-24, WM451, WM35) were purchased from the American Type Culture Collection (Manassas, VA, USA) and incubated with DMEM medium containing 10% fetal bovine serum in an incubator at 37°C. When the cell confluence was over 80%, melanoma cells were seeded in 6-well plates and cultivated for 24 h before transfection. After that, melanoma cells were transfected with TRIM14 knockdown (siRNA-TRIM14) or overexpression (Oe-TRIM14) plasmid and their corresponding negative controls (siRNA-NC or Oe-TRIM14) for the indicated time, and then they were used for the following assays.
4
Investigating NORAD Expression in Melanoma
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Including paraffin‐embedded primary MM (62 cases), and normal skin tissues (20 cases) was purchased from Auragene Co. Ltd (cat No. TC0229; Changsha, China) for in situ hybridization confirmation of NORAD expression. Human melanocytes (HM) and four human MM cell lines (A375, WM451, SK‐MEL‐24, and WM35) purchased from the American Type Culture Collection (Manassas, VA) were cultured in RPMI‐1640 medium (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (Gibco, Carlsbad, CA) in a humidified atmosphere of 5% CO2 at 37°C. Ectopic miR‐205 expression was observed after transfection with either pre‐miR‐205 or anti‐miR‐205 (Genepharma, Shanghai, China) using Lipofectamine 3000 (Invitrogen). Knockdown of NORAD was performed using lentivirus (Lv) containing short hairpin (sh)RNA sequences of NORAD (GeneCopoecia, Guangzhou, China). Cells were cultured in either 6‐well clusters or 96‐well plates for 24 or 48 hours, and then used to experiments or RNA/protein extraction.
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