96 well solid polystyrene microplate

Kit ProcartaPlex Human Coronavirus Ig Total Panel 11-plex was used for antigen study as previously reported using xMAP’s methodology (16 (link)). All plasma samples were incubated in Corning^® 96 Well Solid Polystyrene Microplate together with the standard samples for relative quantification, medium and low control CoV and Assay Buffer as blank. Standard serial dilution was made in PCR 8-Tube Strip. All soluble immunoglobulins (S1 protein for all CoV and for SARS-CoV-2 in addition to the spike trimer -S1 + S2-, RBD and N proteins) are captured with a Bead mix. All washes were carried out with Wash Buffer diluted in deionized water and used a Magnetic 96-Well Separator. Detection antibody was made with Ig total Det Antibody diluted with Detection Ab Diluent (1×). Acquisition was made in Reading Buffer with xMAP^® instruments and the MAGPIX^® System software was used for analysis. All incubation steps were made in agitation with Fisherbrand™ Microplate Vortex Mixers and cover the plate with Plate Cover and Black Microplate Lid.

These studies were performed with the following Luminex kits according to the manufacturer’s instructions: Human Immuno-Oncology Checkpoint marker Panel 1 (EPX14A-15803-901), Human Immuno-Oncology Checkpoint marker Panel 2 (EPX140-15815-901), and Human Immuno-Oncology Checkpoint marker Panel 3 (EPX-100-1582-901). All the serum samples were incubated in a 96-well Solid Polystyrene Microplate (Corning^®, EE.UU.), and during the washing process, a magnetic 96-well separator (Thermo Fisher Scientific, EE.UU.) was used following the manufacturer’s instructions. The acquisition was performed on MAGPIX^® Instrument and xMAP^® component software v. 4.2. All experiments were performed by the same operator according to the manufacturer’s instructions.