The murine monocyte cell line J774A.1 (CLS Cell Lines Service GmbH, Eppelheim, Germany) was cultured in DMEM/F-12 (1 + 1) (+)-L-glutamine (Gibco, Germany) supplemented with 10 vol% fetal calf serum (FCS; Gibco). The murine endothelial cell line SVEC4-10 (American Type Culture Collection, ATCC, 141 CCL81, Rockville, MD) was cultured in DMEM (1x) + GlutaMAXTM-I (Gibco, Germany) supplemented with 10 vol% heat-inactivated FCS. The murine T-lymphocyte (lymphoma) cell line TK-1 (ATCC) was cultured in RPMI 1640 medium supplemented with 10 vol% FCS, 0.1 mM non-essential amino acids, and 0.05 mM 2-mercaptoethanol (Thermo Fisher, Germany). The murine B lymphocyte (myeloma) cell line MOPC-315 (ATCC) was cultured in DMEM (Gibco, Germany) supplemented with 10 vol% FCS. All cells were cultured at 37 °C in 5% CO2 atmosphere.
Dmem f 12 1 1 l glutamine
Manufactured by Thermo Fisher Scientific
Sourced in Germany
DMEM/F-12 (1 + 1) (+)-L-glutamine is a cell culture medium designed for the growth and maintenance of a variety of cell types. It is a combination of Dulbecco's Modified Eagle's Medium (DMEM) and Ham's F-12 Nutrient Mixture in a 1:1 ratio, and it is supplemented with L-glutamine, an essential amino acid.
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Lab products found in correlation
2 protocols using dmem f 12 1 1 l glutamine
1
Murine Cell Line Culture Protocols
2
Murine Macrophage Cell Line Cultivation
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To elucidate the impact of NPs on potential target cells, the murine macrophage cell line J774A.1 (CLS Cell Lines Service GmbH, Eppelheim, Germany) was cultured in DMEM/F-12 (1:1) (+)-l -glutamine (Gibco, Darmstadt, Germany) containing 10% fetal calf serum (FCS; Gibco) at 37 °C in a 5% CO2 atmosphere. When confluency reached approximately a value of 80%, cells were harvested by scraping and used for further experiments as described below.
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