For histopathological examination, three fish from each group per replicate for histopathological studies were removed after treatment periods of 24 h. Hepatocyte tissues were dissected and fixed in Bouin’s fluid for 24 h, and washed with tap water. They were processed through a graded ethanol series, cleared in xylene, and embedded in paraffin. Paraffin samples were sectioned at a thickness of 5 μm, and stained with hematoxylin and eosin (HE) for light microscopic observation. The sections were examined under a light microscope (Olympus Vanox photomicroscope, Japan).
Vanox photomicroscope
Manufactured by Olympus
Sourced in Japan
The Vanox photomicroscope is a high-quality optical instrument designed for microscopic imaging and analysis. It features a compact, durable design and advanced optics for clear, detailed observations. The Vanox provides consistent, reliable performance for a variety of laboratory applications.
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Lab products found in correlation
3 protocols using vanox photomicroscope
1
Histopathological Analysis of Fish Liver
2
Histological Analysis of Rat Liver
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Autopsy samples were taken from the liver of rats in several groups and fixed in 10% formalin saline for 7 days, after completion of fixation, the samples were dehydrated in a series of alcohols, cleared in toluene and then embedded in paraplast (Sherweed Medical Co, USA). Blocks were cut at 5-7 µm. Sections were de-waxed, re-hydrated in a series of alcohols, stained in Harris hematoxylin (Cole Parmer, USA) and counterstained in 1% aqueous eosin (Sigma, USA). Sections were then mounted in DePeX (GURR, BDH, UK) and examined under a light microscope. Representative slides were photographed under an Olympus Vanox photomicroscope (Olympus, USA) (Al-Naqeeb et al., 2003)
3
Histological Assessment of Intestinal Samples
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The intestinal samples (0.5 cm) from duodenum, jejunum and ileum were fixed by immersion in 4% phosphate-buffered paraformaldehyde for 48 h. The samples were then washed in a phosphatebuffered saline solution, dehydrated in an ethanol series and embedded in paraffin. Cross sections were stained with Mayer's hematoxylin and eosin and examined under photomicroscope (Olympus Vanox photomicroscope, Japan) for the histopathological assay. For the analysis of the villus height and crypts depth ten microscopic fields of the duodenum, jejunum and ileum were measured for each bird using a calibrated ocular micrometer.
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