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Zymosan tlrl zyn

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Zymosan (tlrl-zyn) is a cell wall preparation derived from the yeast Saccharomyces cerevisiae. It is composed primarily of beta-glucans and is a ligand for Toll-like receptor 2 (TLR2).

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Lab products found in correlation

Zymosan, by InvivoGen (2 mentions) Dy215, by R&D Systems (2 mentions) Dy271, by R&D Systems (2 mentions) Dy275, by R&D Systems (2 mentions) Dy208, by R&D Systems (2 mentions) Curdlan, by Merck Group (2 mentions) Hkca tlrl hkca, by InvivoGen (1 mentions) L nmma, by Beyotime (1 mentions) Stat3 in 1, by MedChemExpress (1 mentions) Sis17, by MedChemExpress (1 mentions) Jsh 23, by MedChemExpress (1 mentions) Trehalose 6 6 dibehenate, by InvivoGen (1 mentions)

Close spelling variants of this product

Zymosan (68 citations)

3 protocols using zymosan tlrl zyn

1

Immune Response Modulation Protocols

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Heat-killed C. albicans (HKCA; tlrl-hkca), zymosan (tlrl-zyn) and Trehalose-6,6-dibehenate (TDB; tlrl-tdb) were purchased from Invivogen. Mannan (M3640) were purchased from Sigma-Aldrich. The HDAC11 inhibitors FT895 (HY-112285) and SIS17 (HY-128918), SYK inhibitor R406 (HY-12067), NF-κB inhibitor JSH-23 (HY-13982) and STAT3 inhibitor STAT3-IN-1 (HY-100753) were purchased from MedChemExpress. iNOS inhibitors S-methylisothiourea hemisulfate salt (SMT; S0008) and L-NMMA (S0011) were purchased from Beyotime Biotechnology. The SYK inhibitor R406 (S2194) was purchased from Selleck. The antibodies used in this study shown in Supplementary Table 1.
Wu H., Yin X., Zhao X., Wu Z., Xiao Y., Di Q., Sun P., Tang H., Quan J, & Chen W. (2022). HDAC11 negatively regulates antifungal immunity by inhibiting Nos2 expression via binding with transcriptional repressor STAT3. Redox Biology, 56, 102461.
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2

Zymosan and Curdlan Induced Cytokine Secretion

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Cells were seeded in 12 well plates at a density of 7.5×105 (SW480) cells/well. After overnight incubation, the medium was replaced with fresh medium and Zymosan (tlrl-zyn, InvivoGen, San Diego, CA) or Curdlan (C7821 Sigma-Aldrich, St. Louis, MO). To avoid possible contaminating with endotoxin, we used endotoxin-free (<0.001 EU) Zymosan or Curdlan. Where indicated the SYK inhibitors [3-(1-methyl-1H-indol-3-yl-methylene)-2-oxo-2,3-dihydro-1H-indole-5-sulfonamide], (*574711, CAS 622387–85-3 Calbiochem, Merck-Millipore, Darmstadt, Germany) and R406 (Invivogen), were added 1 hour prior to the addition of Zymosan. All treatments were performed in duplicates/triplicate. After 20 hours, supernatants were collected, and maintained at −70ºC until analysis. IL-8, CCL2, CXCL1 and GM-CSF concentrations were determined using ELISA (DY208, DY271, DY215 and DY275 respectively from R&D systems) according to the manufacturer’s instructions.
Wang L., Aschenbrenner D., Zeng Z., Cao X., Mayr D., Mehta M., Capitani M., Warner N., Pan J., Wang L., Li Q., Zuo T., Cohen-Kedar S., Lu J., Ardy R.C., Mulder D.J., Dissanayake D., Peng K., Huang Z., Li X., Wang Y., Wang X., Li S., Bullers S., Gammage A.N., Warnatz K., Schiefer A.I., Krivan G., Goda V., Kahr W.H., Lemaire M., Lu C.Y., Siddiqui I., Surette M.G., Kotlarz D., Engelhardt K.R., Griffin H.R., Rottapel R., Decaluwe H., Laxer R.M., Proietti M., Hambleton S., Elcombe S., Guo C.H., Grimbacher B., Dotan I., Ng S.C., Freeman S.A., Snapper S.B., Klein C., Boztug K., Huang Y., Li D., Uhlig H.H, & Muise A.M. (2021). Gain-of-function variants in SYK cause immune dysregulation and systemic inflammation in humans and mice. Nature genetics, 53(4), 500-510.
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3

Zymosan and Curdlan Induced Cytokine Secretion

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Cells were seeded in 12 well plates at a density of 7.5×105 (SW480) cells/well. After overnight incubation, the medium was replaced with fresh medium and Zymosan (tlrl-zyn, InvivoGen, San Diego, CA) or Curdlan (C7821 Sigma-Aldrich, St. Louis, MO). To avoid possible contaminating with endotoxin, we used endotoxin-free (<0.001 EU) Zymosan or Curdlan. Where indicated the SYK inhibitors [3-(1-methyl-1H-indol-3-yl-methylene)-2-oxo-2,3-dihydro-1H-indole-5-sulfonamide], (*574711, CAS 622387–85-3 Calbiochem, Merck-Millipore, Darmstadt, Germany) and R406 (Invivogen), were added 1 hour prior to the addition of Zymosan. All treatments were performed in duplicates/triplicate. After 20 hours, supernatants were collected, and maintained at −70ºC until analysis. IL-8, CCL2, CXCL1 and GM-CSF concentrations were determined using ELISA (DY208, DY271, DY215 and DY275 respectively from R&D systems) according to the manufacturer’s instructions.
Wang L., Aschenbrenner D., Zeng Z., Cao X., Mayr D., Mehta M., Capitani M., Warner N., Pan J., Wang L., Li Q., Zuo T., Cohen-Kedar S., Lu J., Ardy R.C., Mulder D.J., Dissanayake D., Peng K., Huang Z., Li X., Wang Y., Wang X., Li S., Bullers S., Gammage A.N., Warnatz K., Schiefer A.I., Krivan G., Goda V., Kahr W.H., Lemaire M., Lu C.Y., Siddiqui I., Surette M.G., Kotlarz D., Engelhardt K.R., Griffin H.R., Rottapel R., Decaluwe H., Laxer R.M., Proietti M., Hambleton S., Elcombe S., Guo C.H., Grimbacher B., Dotan I., Ng S.C., Freeman S.A., Snapper S.B., Klein C., Boztug K., Huang Y., Li D., Uhlig H.H, & Muise A.M. (2021). Gain-of-function variants in SYK cause immune dysregulation and systemic inflammation in humans and mice. Nature genetics, 53(4), 500-510.
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