NCI-H1703 was seeded at 400 cells/well in a Costar ultra-low attachment microplate (Corning, New York, NY, USA) by using the same DMEM medium used for monolayer culture. After an overnight incubation, matrigel (final concentration at 2.5% (v/v)) was added gently toward the center of the well to facilitate the compact formation of a spherical structure. The spheroid was optimized to grow to 300–400 μm in diameter four days post-generation. The diameter of the spheroid was measured by using Zen 2 pro software (Carl Zeiss, Oberkochen, Germany).
Costar ultra low attachment microplate
Manufactured by Corning
Sourced in United States
The Costar ultra-low attachment microplate is a laboratory equipment product designed for cell culture applications. The microplate features a unique surface treatment that minimizes cell attachment, promoting the formation of suspension cultures and spheroid structures.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using costar ultra low attachment microplate
1
Spheroid Formation and Characterization
2
3D Spheroid Formation from NCI-H1703 Cells
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NCI-H1703 was seeded at 400 cells/well in a Costar ultra-low attachment microplate (Corning Inc., Corning, NY, USA) by using the same DMEM medium used for monolayer culture. After an overnight incubation, Matrigel (final concentration at 2.5% (v/v), Corning Inc.) was added gently towards the center of the well to facilitate compact formation of spherical structure. The spheroid was optimized to grow to 300–400 μm in diameter four days post-generation. The diameter of spheroid was measured by using Zen 2 pro software (Carl Zeiss).
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