Luciferase assays were performed using the dual-luciferase reporter assay system on a GloMax R96 Microplate Luminometer (Promega). pmirGLO dual-luciferase expression vector reporter (Promega, Madison, WI) was used to clone portions of 3′ untranslated regions of the investigated mRNAs. PcDNA3 was used to balance all amounts to a total of 1 µg DNA per condition.
Pmirglo dual luciferase expression vector reporter
Manufactured by Promega
The PmirGLO Dual-Luciferase Expression Vector is a reporter system that allows for the simultaneous measurement of firefly and Renilla luciferase activities within a single sample. The vector contains both firefly and Renilla luciferase genes, enabling the monitoring of gene expression and regulation.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using pmirglo dual luciferase expression vector reporter
1
Dual-luciferase Reporter Assays
2
Cloning 3' UTR Regions Using Dual-Luciferase
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pmirGLO dual-luciferase expression vector reporter (Promega, Madison, WI) was used to clone portions of 3′ untranslated regions of the investigated mRNAs. XhoI and SalI restriction enzymes were used.
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