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Lipid peroxidation mda assay

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The Lipid Peroxidation MDA Assay is a laboratory equipment product used to measure the level of malondialdehyde (MDA), a biomarker for oxidative stress. The assay is designed to quantify MDA, which is a byproduct of lipid peroxidation, a process that occurs when free radicals damage cell membranes. The assay provides a means to assess the degree of oxidative stress in biological samples.

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Close spelling variants of this product

MDA) lipid peroxidation microplate assay Lipid Peroxidation [malondialdehyde (MDA)] assay kit Lipid Peroxidation (MDA) Assay Kit

2 protocols using lipid peroxidation mda assay

1

Lipid Peroxidation Quantification in Brain

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10 mg of brain were homogenized in 300 µL of Malondialdehyde (MDA) Lysis Buffer, and the Lipid Peroxidation MDA Assay (Sigma-Aldrich) was used to detect the concentration of lipid peroxidation, according to the manufacturer’s instructions. Absorbance was measured at 532 nm with the iMark™ Microplate Absorbance Reader.
Nuzzo D., Amato A., Picone P., Terzo S., Galizzi G., Bonina F.P., Mulè F, & Di Carlo M. (2018). A Natural Dietary Supplement with a Combination of Nutrients Prevents Neurodegeneration Induced by a High Fat Diet in Mice. Nutrients, 10(9), 1130.
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2

Brain and Plasma Lipid Peroxidation Assay

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To detect the concentration of brain lipid peroxidation, 10 mg of frozen homogenate brain tissue was resuspended in 300 μL of malondialdehyde (MDA) lysis buffer, and the lipid peroxidation MDA assay (Sigma-Aldrich, Milan, Italy) was used according to the manufacturer’s instructions. Absorbance was measured at 532 nm by using the GloMax® Discover multimode plate reader. To detect the concentration of plasma lipid peroxidation, 20 µL of plasma was processed as described above.
Nuzzo D., Galizzi G., Amato A., Terzo S., Picone P., Cristaldi L., Mulè F, & Di Carlo M. (2020). Regular Intake of Pistachio Mitigates the Deleterious Effects of a High Fat-Diet in the Brain of Obese Mice. Antioxidants, 9(4), 317.
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