RNA was isolated from frontal cortex tissue of nine brains exhibiting normal hnRNP K mislocalisation (5 controls, 4 FTLD-TDPA) and 12 brains exhibiting mislocalised hnRNP K (8 FTLD-TDP A, 1 FTLD-TDP B, 1 FTLD-TDP C, 1 FTLD-ni and 1 control) as indicated in Table 1. 50 mg of flash-frozen tissue was homogenised in 700 µL of Qiazol (Qiagen) using a TissueRuptor II (Qiagen). Chloroform was added and RNA subsequently extracted following the spin-column protocol from the miRNeasy kit with DNAse digestion (Qiagen). RNA was eluted off the column in 50 µL of RNAse-free water. RNA quantity and quality were evaluated using a spectrophotometer.
Mirneasy kit with dnase digestion
Manufactured by Qiagen
Sourced in Germany
The MiRNeasy kit with DNAse digestion is a laboratory equipment product designed for the purification of microRNA (miRNA) and total RNA from various sample types, including cells, tissues, and body fluids. The kit incorporates a DNase digestion step to eliminate genomic DNA contamination, ensuring the purity of the extracted RNA.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mirneasy kit with dnase digestion
1
Isolation of RNA from Frontal Cortex
2
HUVECs Transcript Analysis via qPCR
Check if the same lab product or an alternative is used in the 5 most similar protocols
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HUVECs were plated and treated with 1000 µg mL−1 BE or combinations of bzATP and AZ11645373 as previously described. The cells were harvested with 700 µL QIAzol (Qiagen, Germany), incubated for 5 min in RT, and frozen at −80 °C. RNA isolation was performed with miRNeasy kit with DNase digestion (Qiagen, Germany) as per manufacturer's instructions. High Capacity RNA to cDNA kit (Thermo Fisher Scientific, US) was used for reverse transcription. The mRNA levels for P2X7, Rab27b, Rab27a, SMPD1, and ARF6 with GAPDH as a reference gene were assessed using TaqMan Fast Universal PCR Master Mixes (Thermo Fisher Scientific, US) and 20 ng template as per manufacturer's instructions.
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