The antibodies recognizing TNIK (1:1000 for western blotting [WB], #612210, RRID: AB_399573) and phosphoserine/threonine residues (1:1000 for WB, #612548, RRID: AB_399843) were purchased from BD Biosciences (San Jose, CA, USA). The Arc antibody (1:2000 for WB, #156 003, RRID: AB_887694) was from Synaptic Systems (Goettingen, Germany) while the horseradish peroxidase (HRP)-conjugated FLAG (1:1000 for WB, A8592, RRID: AB_439702), FLAG M2 (1:1000 for WB, F1804, RRID: AB_262044), β-actin (1:100,000 for WB, A1978, RRID: AB_476692), and GAPDH (1:100,000 for WB, AB2302, RRID: AB_10615768) antibodies were from Sigma-Aldrich (St. Louis, MO, USA). The antibodies detecting GST (1:1000 for WB, #2625, RRID: AB_490796) and Myc-tag (1:1000 for WB, #2276, RRID: AB_331783) were acquired from Cell Signaling Technology (Beverly, MA, USA) whereas the drebrin antibody (1:500 for WB, sc-374269, RRID: AB_10990108) was from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Finally, the GFP (1:1000 for ICC, A10262, RRID: AB_2534023) and cross-absorbed HRP-conjugated secondary antibodies were from Thermo Fisher Scientific (Waltham, MA, USA).
AK-7 was purchased from Tocris Bioscience (Bristol, UK), oxamflatin from Santa Cruz Biotechnology, and KY-05009 from Sigma-Aldrich. The inactive analog G883–2176 was obtained from commercial sources (Molport, Beacon, NY, USA).
AK-7 was purchased from Tocris Bioscience (Bristol, UK), oxamflatin from Santa Cruz Biotechnology, and KY-05009 from Sigma-Aldrich. The inactive analog G883–2176 was obtained from commercial sources (Molport, Beacon, NY, USA).