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The method for cell lysis is described elsewhere (Mäki-Jouppila et al, 2015 (link)). Lysed protein samples were run on 4–20% gels (Bio-Rad) and then transferred with semi-dry transfer equipment (Bio-Rad) to nitrocellulose membrane. 5% milk/TBS-T, 5% BSA/TBS-T and Odyssey blocking buffer (LI-COR Biotechnology, Lincoln, NE, USA)/TBS-T (1 : 1) were used as blocking agents. The primary antibodies used were mouse anti-Rassf1a (1 : 500; ab23950, Abcam, Cambridge, UK or SM6017, Acris antibodies GmbH, Herford, Germany), rabbit anti-STX16 (1 : 750; HPA041019, Atlas antibodies, Stockholm, Sweden) and mouse anti-GAPDH (1 : 30 000–50 000; mAb 6C5, Advanced ImmunoChemical Inc., Long Beach, CA, USA, or HyTest Ltd, Turku, Finland). Primary antibodies were diluted into TSB-T or in the case of Rassf1a antibody, into TBS-T/Odyssey blocking buffer (5 : 1) when infrared detection system was used. Secondary antibodies (1 : 5000 in TBS-T) and detection methods used are described in previous publication (Tambe et al, 2016 (link)) with the addition of HRP-linked anti-rabbit IgG (Cell Signalling Technology, Danvers, MA, USA) used also as a secondary antibody.