Five hundred milligrams of F127, 10 mg of F68, 10 mg of HA (1500–2500 kDa), and 2 mL of Bor/RB-M were stirred strongly in an ice-water bath for 4 h and stored in a refrigerator for later use. A similar process was used to prepare Sd III-M@TRG and Bor/C6-M@TRG. The kinetics of dissolution of the gel was observed using a film-free dissolution method as reported previously [23 (link)]. Briefly, 1 mL of Sd III-M@TRG solution was injected into 20 mL of phosphate-buffered saline (PBS) at 37 °C, followed by shaking at 100 rpm and acquiring the pictures at the predetermined time intervals. The rheology of Bor/RB-M@TRG and Blank TRG was analyzed by using an Anton Palmer MCR302 rheometer (TA Instruments, Graz, Austria) in the oscillatory mode. The samples were placed on a parallel plate (40 mm diameter) with a gap of 31 mm for measurements. The storage moduli (G′) and loss moduli (G″) were monitored as a function of temperature, at a frequency of 1 Hz and a strain of 1%. The morphology of the hydrogels was studied with scanning electron microscopy (SEM) by using the manipulation protocol.
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