We used a free-ranging experimental population of A. femoralis that was introduced in 2012 on a small (~ 5 ha) river island in tropical lowland rainforest located in the Nouragues Nature Reserve, French Guiana (4°02′ N 52°41′ W;36 (link)–38 (link)). The study was conducted between April–May 2022 during the reproductive period of the species25 (link) and all tests were performed during its main calling activity (1400–1900 h,39 (link)). We surveyed the population every day aiming to sample all adult males present on the island and caught frogs using a transparent plastic bag to minimize direct contact and stress. We took dorsal photographs on mm-paper to determine body size (snout urostyle length) using the software Image J 1.52a40 , and ventral photographs to identify frogs via their distinct belly patterns. We later confirmed identifications from the field with the pattern matching software Wild-ID41 (link). In total we caught 76 males with an average body size of 2.9 cm (± 0.1 SD).
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