Lyve1+/GFPCre mice were obtained from the Jackson Laboratory (stock no. 012601) (31 (link)) and were back-crossed to NMRI background for at least 6 generations. Prox1+/− (Prox1+/LacZ, Prox1+/GFPCre) mice have been reported previously (62 (link), 63 (link)). Lepob/ob mice (referred to as ob/ob mice herein) were also obtained from the Jackson Laboratory and backcrossed to the NMRI background. Prox1+/Flox and Jojo-Prox1 mice have been described previously (7 (link), 33 (link), 34 (link)).
Protocol detail
Generation and Characterization of Transgenic Mouse Lines
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Lacz
Mice
Nmri
Corresponding Organization : St. Jude Children's Research Hospital
Other organizations : ETH Zurich
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Variable analysis
independent variables
- Genotype of mice (e.g., Lyve1+/GFPCre, Prox1+/-, Lep ob/ob)
- Not explicitly mentioned
- Genetic background (NMRI) of the mice
- Number of generations the mice were back-crossed (at least 6)
- Positive control: Prox1+/- (Prox1+/LacZ, Prox1+/GFPCre) mice
- Negative control: Not explicitly mentioned
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