Cell proliferation ability assay referred to the method as we previously described [61 (link)]. Cells from the logarithmic growth phase were seeded in 48-well plates with 50,000 per well, and the cells were treated at the indicated concentration of honokiol after 24 h, with inhibitors, treated one hour in advance. After 24 h of Honokiol treatment, cells were collected and counted by a cell counter (CountStar, Shanghai, China).
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