Conditional Knockout of ATG5 in T cells

C57BL/6 (B6, H-2^b) and BALB/c (H-2^d) mice were purchased from the Jackson Laboratory (Bar Harbor, ME, USA) and Charles River Laboratories (Wilmington, MA, USA). Previously described ^{29 (link)
15 (link)} B6-background ATG5^flox/flox mice (B6.129S-ATG5, #RBRC02975, Riken RBC, Ibaraki, Japan) were bred to Lck-Cre (B6 Cg-Tg(Lck-Cre)1Cwi N9) transgenic mice (Taconic, #004197, Hudson, New York). All animals were cared for according to regulations reviewed and approved by the University Committee on Use and Care of Animals of the University of Michigan, based on University Laboratory Animal Medicine guidelines.

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Oravecz-Wilson K., Rossi C., Zajac C., Sun Y., Li L., Decoville T., Fujiwara H., Kim S., Peltier D, & Reddy P. (2020). ATG5-dependent autophagy uncouples T cell proliferative and effector functions and separates graft-versus-host disease from graft- versus-leukemia. Cancer research, 81(4), 1063-1075.

Publication 2020

BALB/c (H-2d) B6 Cg-Tg(Lck-Cre)1Cwi N9)

Animals Laboratory animal Medicine Mice River Transgenic mice

Corresponding Organization : University of Michigan–Ann Arbor

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Variable analysis

independent variables

Mouse strains: C57BL/6 (B6, H-2^b) and BALB/c (H-2^d) mice

dependent variables

Not explicitly mentioned

control variables

B6-background ATG5^flox/flox mice (B6.129S-ATG5^tm1Myok)
Lck-Cre (B6 Cg-Tg(Lck-Cre)1Cwi N9) transgenic mice

controls

Positive control: B6-background ATG5^flox/flox mice
Negative control: Lck-Cre (B6 Cg-Tg(Lck-Cre)1Cwi N9) transgenic mice

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