Molecular Signaling Pathway Analysis

Cells were lysed in lysis buffer in the presence of protease inhibitors and phosphatase inhibitors. Cell lysates were then subjected to western blotting as previously described^{41 (link)}. Primary antibodies were used as follows: p-ErbB2 (Y1221/1222) (2243#), ErbB2 (2165#), DEPTOR (11816#), p-AKT (S473) (4060#), p-AKT (T308) (4056#), t-AKT (4691#), p-S6K1 (T389) (9234#), p-ERK (T202/Y204) (9101#), t-ERK (4696#), mTOR (2972#), PARP (9532#), and LC3B (2775#) (Cell Signaling Technology); t-S6K (sc-230#), and ErbB2 (sc-33684#) (Santa Cruz); Tubulin (T9026#), LC3B (L7543#), and ACTIN (A5441#) (Sigma); p62 (PM045#) (Medical & Biological Laboratories).

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Bi Y., Gong L., Liu P., Xiong X, & Zhao Y. (2021). Nuclear ErbB2 represses DEPTOR transcription to inhibit autophagy in breast cancer cells. Cell Death & Disease, 12(4), 397.

Publication 2021

p-ErbB2 ErbB2 DEPTOR p-AKT t-AKT p-S6K1 p-ERK t-ERK Tubulin ACTIN

Actin Antibodies Biological Buffer Cell Erbb2 Inhibitors Mtor Phosphatase Protease inhibitors Tubulin

Corresponding Organization :

Other organizations : Zhejiang University, First Affiliated Hospital Zhejiang University

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Variable analysis

independent variables

Lysis buffer
Protease inhibitors
Phosphatase inhibitors

dependent variables

Protein expression levels of p-ErbB2 (Y1221/1222)
ErbB2
DEPTOR
P-AKT (S473)
P-AKT (T308)
T-AKT
P-S6K1 (T389)
P-ERK (T202/Y204)
T-ERK
T-S6K
Tubulin

control variables

Cell lysates subjected to western blotting as previously described in the referenced study

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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