Real-Time RT-qPCR and RNA-seq Analysis Protocol

All analysis for real time RT-qPCR and RNA-seq are presented as the mean±S.E.M [15 (link)]. Each experiment was performed at least three times and subjected to statistical analysis. One-way analysis of variance (ANOVA) was first performed to determine differences among groups (*: p<0.05; **: p<0.01). Fisher’s post hoc test was then performed to determine significant differences between pairs. Values of p<0.05 and p<0.01 were considered significant. Statistical tests were performed using Stat View software version 5.0 (SAS Institute Inc., Cary, NC, USA).

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Choi Y.J., Kang M.H., Hong K, & Kim J.H. (2019). Tubastatin A inhibits HDAC and Sirtuin activity rather than being a HDAC6-specific inhibitor in mouse oocytes. Aging (Albany NY), 11(6), 1759-1777.

Publication 2019

Stat View software version 5.0

Rna seq

Corresponding Organization :

Other organizations : Konkuk University

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Variable analysis

independent variables

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Real time RT-qPCR results
RNA-seq results

control variables

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