Albumin synthesis was measured in cell culture supernatants on day 3 after 24 h in serum- free EGM-2 medium using an Albumin Blue 580 Fluorescence Assay as previously described31 (link). Data was normalized to the number of viable C3As seeded (day 0). CYP3A4 enzyme activity was assessed using a non-lytic luminescence assay (P450-Glo, Promega) following the manufacturer’s instructions.
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