Rapeseed Genomic DNA Extraction and SNP Analysis

Genomic DNA was extracted from 100 mg of leaf tissue from 3 young seedlings for each of the 520 rapeseed accessions using the Cetyltrimethylam-monium bromide (CTAB) method. The SNP analysis was performed in the National Key Laboratory of Crop Genetic Improvement, National Subcenter of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan, China, according to the manufacture’s protocol [39 ]. The SNP data were analyzed according to the previous used protocols [18 (link)] by Illumina BeadStudio genotyping software.

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Qu C.M., Li S.M., Duan X.J., Fan J.H., Jia L.D., Zhao H.Y., Lu K., Li J.N., Xu X.F, & Wang R. (2015). Identification of Candidate Genes for Seed Glucosinolate Content Using Association Mapping in Brassica napus L.. Genes, 6(4), 1215-1229.

Publication 2015

BeadStudio genotyping software

Bromide Crop Genomic Leaf Seedlings Tissue

Corresponding Organization : Southwest University

Other organizations : University of Saskatchewan

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Variable analysis

independent variables

Amount of leaf tissue used for DNA extraction (100 mg)

dependent variables

SNP (Single Nucleotide Polymorphism) data

control variables

Leaf tissue from 3 young seedlings for each of the 520 rapeseed accessions
Cetyltrimethylam-monium bromide (CTAB) method for DNA extraction
SNP analysis performed at the National Key Laboratory of Crop Genetic Improvement, National Subcenter of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan, China
Illumina BeadStudio genotyping software used for SNP data analysis

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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