Radiolabeling of PSMA Ligand NG001 with Lead-212

The PSMA ligand NG001 was supplied as HPLC purified and dried trifluoroacetic acid salt (purity of ≥98%) by MedKoo Biosciences (Morrisville, NC, USA). NG001 dissolved in 0.5 M ammonium acetate in 0.1 M HCl was labelled with ²¹²Pb using a liquid ²²⁴Ra/²¹²Pb generator solution, as previously described [25 (link),68 (link)]. The ²¹²Pb-NG001 was purified using PD Minitrap G-10 columns prepacked with Sephadex G-10 resin (GE Healthcare Bio-Sciences AB, Uppsala, Sweden) to remove free ²²⁴Ra to a level below 0.8%. The radiochemical purity (RCP) of the radioligand was measured by thin-layer chromatography, and radioligands with RCP >95% were used for the experiments.

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Stenberg V.Y., Larsen R.H., Ma L.W., Peng Q., Juzenas P., Bruland Ø.S, & Juzeniene A. (2021). Evaluation of the PSMA-Binding Ligand 212Pb-NG001 in Multicellular Tumour Spheroid and Mouse Models of Prostate Cancer. International Journal of Molecular Sciences, 22(9), 4815.

Publication 2021

PD Minitrap G-10 columns Sephadex G-10 resin

Ammonium acetate Hplc Ligand Radiochemical Resin Salt Sephadex g 10 Thin layer chromatography Trifluoroacetic acid

Corresponding Organization : Oslo University Hospital

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Variable analysis

independent variables

NG001 dissolved in 0.5 M ammonium acetate in 0.1 M HCl

dependent variables

Radiochemical purity (RCP) of the radioligand

control variables

PSMA ligand NG001 supplied as HPLC purified and dried trifluoroacetic acid salt (purity of ≥98%)
Labelling of NG001 with ²¹²Pb using a liquid ²²⁴Ra/²¹²Pb generator solution
Purification of ²¹²Pb-NG001 using PD Minitrap G-10 columns prepacked with Sephadex G-10 resin to remove free ²²⁴Ra to a level below 0.8%

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