Cytokine Stimulation Titration Analysis

Titration analyses of cytokine stimulation were performed as described in the accompanying study (26 (link)). To follow up on those studies, recombinant human (rh) TNFα (#300-01A, PeproTech, Rocky Hill, NJ) and rh IL-1β (#200-01B, PeproTech) were used in the following concentrations: MDA-MB-231 cells, MCF-7 cells, MSCs and CAFs: TNFα 10 ng/ml, IL-1β 350 pg/ml; MDA-MB-468 cells: TNFα 50 ng/ml, IL-1β 500 pg/ml; BT-549 cells: TNFα 25 ng/ml, IL-1β 350 pg/ml.

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Liubomirski Y., Lerrer S., Meshel T., Morein D., Rubinstein-Achiasaf L., Sprinzak D., Wiemann S., Körner C., Ehrlich M, & Ben-Baruch A. (2019). Notch-Mediated Tumor-Stroma-Inflammation Networks Promote Invasive Properties and CXCL8 Expression in Triple-Negative Breast Cancer. Frontiers in Immunology, 10, 804.

Publication 2019

rh) TNFα rhIL-1β IL-1β

Cafs Cells Cytokine Human Il 1β Mcf 7 cells Mda mb 231 cells Titration Tnfα

Corresponding Organization : Tel Aviv University

Other organizations : Heidelberg University, German Cancer Research Center

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Variable analysis

independent variables

Recombinant human (rh) TNFα concentration
Recombinant human (rh) IL-1β concentration

dependent variables

Cytokine stimulation response

control variables

Cell lines used: MDA-MB-231, MCF-7, MSCs, CAFs, MDA-MB-468, BT-549

controls

No positive or negative controls were explicitly mentioned.

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