Mice for Neuroscience Research

All experiments involving animals were approved by the IIT Animal Welfare Body, by the National Council on Animal Care of the Italian Ministry of Health (authorizations #34/2015-PR, #1084/2020-PR), and by the Institutional Animal Care and Use Committee at the Weizmann Institute of Science, and carried out in accordance with the guidelines established by the European Communities Council Directive. The following mouse lines were used for this study: C57BL/6J mice (IMSR Cat# JAX:000664, RRID:IMSR_JAX:000664; Charles River, Calco, Italy and Envigo, Rehovot, Israel) and STOCK Sst^tm2.1(cre)Zjh/J (IMSR Cat# JAX:013044, RRID:IMSR_JAX:013044 - called SST-cre line - Jackson Laboratory, Bar Harbor, USA). Mice were housed in individually ventilated cages under a 12 hr light:dark cycle (≤5 animals per cage). Access to food and water was ad libitum. All the in vivo experiments were performed on urethane-anesthetized young-adult animals (3–16 weeks old, either sex), as described previously (Forli et al., 2018 (link)). The number of animals used for each experimental dataset is specified in the text or in the corresponding Figure legend.