Measurement of Serum IL-6 as Inflammation Proxy

IL-6 had been previously measured in frozen serum samples from PEARL patients using an enzyme-linked immunoassay (Quantikine^®HS ELISA, R&D Systems^®) according to the manufacturer's instructions as previously described (21 (link)). The biobank of La Princesa University Hospital—Health Research Institute (ISS-IP) provided serum for this previous study. In the present work, we have used these previous serum IL-6 measurements as a surrogate for inflammation in the PEARL study, to analyze their relationship with the different indices studied.
IL-6 was measured as a surrogate for inflammation (14 (link)) only in the PEARL study, using an enzyme-linked immunoassay (Quantikine^®HS ELISA, R&D Systems^®) according to the manufacturer's instructions.

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Rodriguez-García S.C., Montes N., Ivorra-Cortes J., Triguero-Martinez A., Rodriguez-Rodriguez L., Castrejón I., Carmona L, & González-Álvaro I. (2021). Disease Activity Indices in Rheumatoid Arthritis: Comparative Performance to Detect Changes in Function, IL-6 Levels, and Radiographic Progression. Frontiers in Medicine, 8, 669688.

Publication 2021

Quantikine®HS ELISA

Elisa Enzyme immunoassay Frozen Inflammation Patients Serum

Corresponding Organization : Hospital Universitario de La Princesa

Other organizations : Hospital Universitari i Politècnic La Fe, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos, Hospital General Universitario Gregorio Marañón, Instituto de Salud Musculoesquelética

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

IL-6 levels (as a surrogate for inflammation)

control variables

None explicitly mentioned

controls

Positive control: The authors mention that IL-6 was measured using a previously validated enzyme-linked immunoassay (Quantikine®HS ELISA, R&D Systems®) according to the manufacturer's instructions.
Negative control: No negative controls were explicitly mentioned in the provided information.

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