Chloroplast Fractionation and Immunoblotting
Corresponding Organization : Western Sydney University
Other organizations : China National Rice Research Institute, Zhejiang University, Qingdao Agricultural University
Variable analysis
- Isolation of intact chloroplasts and thylakoid, stroma and envelop subfractions
- Protein levels detected by Western blotting using specific antibodies
- Standard techniques used for Western blotting
- Enhanced chemiluminescence kit (Invitrogen) used for immunodetection of proteins on a polyvinylidene fluoride (PVDF) membrane
- ChemiDoc Touch imaging system (Bio‐Rad) used for analysis
- No positive or negative controls explicitly mentioned
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!