Influenza Virus RNA Quantification

Viral RNA quantification was performed in nasal swab samples using the NucleoSpin RNA isolation kit (MACHEREY-NAGELGmbH&CoKG, Düren, Germany) following the manufacturer’s instructions. Subsequently, a TaqMan RT-qPCR designed to detect influenza viruses (IVs) using the PCR primers and hydrolysis probe already described [45 (link)] was run in a Fast7500 equipment (Applied Biosystems, CA, USA) with the conditions already set and described [14 (link)].

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Sisteré-Oró M., López-Serrano S., Veljkovic V., Pina-Pedrero S., Vergara-Alert J., Córdoba L., Pérez-Maillo M., Pleguezuelos P., Vidal E., Segalés J., Nielsen J., Fomsgaard A, & Darji A. (2019). DNA vaccine based on conserved HA-peptides induces strong immune response and rapidly clears influenza virus infection from vaccinated pigs. PLoS ONE, 14(9), e0222201.

Publication 2019

NucleoSpin RNA isolation kit Fast7500 equipment

Hydrolysis Influenza viruses Isolation Nasal Primers Viral rna

Corresponding Organization : Statens Serum Institut

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Variable analysis

independent variables

RNA isolation kit

dependent variables

Viral RNA quantification

control variables

Nasal swab samples
TaqMan RT-qPCR detection of influenza viruses
PCR primers and hydrolysis probe described in reference [45]
PCR conditions described in reference [14]

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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