Vesicular Stomatitis Virus-based Vaccine Vectors

d-(+)-Trehalose dihydrate, d-mannitol, and dextran (M_r 40,000 kDa) were purchased from Sigma-Aldrich (Ontario, Canada). Recombinant Vesicular Stomatitis Virus-based vaccine vectors (VSVAg85A and VSVGFP) were produced in the vector facility of McMaster Immunology Research Centre as described previously^{1 (link)}. The vaccine vector is replication-competent containing the VSV wild-type glycoprotein.

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Toniolo S.P., Afkhami S., D’Agostino M.R., Lichty B.D., Cranston E.D., Xing Z, & Thompson M.R. (2020). Spray dried VSV-vectored vaccine is thermally stable and immunologically active in vivo. Scientific Reports, 10, 13349.

Publication 2020

d-(+)-Trehalose dihydrate d-mannitol

Dextran Glycoprotein Mannitol Recombinant vaccine Replication Trehalose Vaccine Vector Vesicular stomatitis virus

Corresponding Organization :

Other organizations : McMaster University

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Variable analysis

independent variables

d-(+)-Trehalose dihydrate
d-mannitol
Dextran (M_r 40,000 kDa)

dependent variables

Unspecified

control variables

Unspecified

controls

Positive control: Recombinant Vesicular Stomatitis Virus-based vaccine vectors (VSVAg85A and VSVGFP) produced in the vector facility of McMaster Immunology Research Centre
Negative control: Not specified

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