Standard procedure of fission yeast manipulation was followed [80 (link),81 (link)]. Cell growth was carried out in YEA media (3% glucose, 0.5% yeast extract, 75 mg/L adenine) (Sigma-Aldrich, St Louis, MO, USA). Solid media plates contained 2% Bacto-agar (BD Bioscience, Franklin Lakes, NJ, USA). Genetic crosses were performed by mixing haploid cells of opposite mating types on SPA plates and meiotic progenies dissected using automated dissection microscope MSM400 (Singer Instruments, Somerset, UK) as previously described [21 (link),82 (link),83 (link)]. MER strains were obtained from previous screens that examined sensitivity to multiple cytotoxic agents, chemotherapeutic drugs and cation [31 (link),33 (link),34 (link),35 (link)]. These are haploid prototrophic strains and arise from backcrossing commercially purchased single-gene knockout library strains (Bioneer, Daejeon, Republic of Korea) with prototrophic WT strains. Gene disruption was confirmed via PCR using gene-specific primers as described previously [31 (link)]. All strains were stored as a frozen stock in glycerol at −80 °C.
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