Biochemical Analysis of Blood Samples

Biochemical analysis of blood samples was performed as described previously (13 (link)). In short, blood samples were collected into EDTA and serum tubes and were centrifuged for 15 minutes at 1000g at 4 °C and 21 °C, respectively. Aliquots were immediately frozen in liquid nitrogen and subsequently stored at –80 °C until analysis. Plasma glucose, FFAs, TGs, and total cholesterol were analyzed with standard enzymatic methods (ABX Pentra 400 autoanalyzer, Horiba ABX). Plasma insulin concentrations were analyzed with commercially available radioimmunoassay (RIA) kits (Human insulin–specific RIA, Millipore Corp, Billerica, Millipore catalog No. EZHI-14K, RRID: AB_2800327). Plasma concentrations of IL-6 (Meso Scale Discovery, catalog No. K151B3S, RRID: AB_2909464), RBP4 (R&D Systems, catalog No. DRB400, RRID: AB_2813876) were determined by enzyme-linked immunosorbent assay. Serum ACE activity was measured with a standard enzymatic assay (Bühlmann, catalog No. KK-ACK).

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Qiao Q., Bouwman F.G., van Baak M.A., Roumans N.J., Vink R.G, & Mariman E.C. (2022). Plasma Levels of Triglycerides and IL-6 Are Associated With Weight Regain and Fat Mass Expansion. The Journal of Clinical Endocrinology and Metabolism, 107(7), 1920-1929.

Publication 2022

ABX Pentra 400 autoanalyzer Human insulin–specific RIA

Blood Blood analysis Cholesterol Edta Enzymatic Enzymatic assay Enzyme linked immunosorbent assay Ffas Frozen Glucose Human Insulin Nitrogen Plasma Rbp4 Serum

Corresponding Organization : Maastricht University Medical Centre

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Variable analysis

independent variables

Centrifugation time (15 minutes)
Centrifugation speed (1000g)
Centrifugation temperature (4 °C and 21 °C)

dependent variables

Plasma glucose
Plasma FFAs
Plasma TGs
Plasma total cholesterol
Plasma insulin concentrations
Plasma IL-6 concentrations
Plasma RBP4 concentrations
Serum ACE activity

control variables

Blood sample collection into EDTA and serum tubes
Aliquot storage at -80 °C until analysis

controls

No positive or negative controls were explicitly mentioned.

Annotations

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