Enzymatic Conversion of Cellulosic Biomass

In this experiment the chemicals and reagents have been chosen in such a way that is cost effective and serve the purpose well. Also, the reagents have been chosen according to the purpose, quality, price and availability. Enzymes α-amylase, glucoamylase and glucose isomerase were purchased from Sigma (Switzerland), Tokyo chemical industry (Japan) and Noor enzyme (India) respectively. Corn starch, D-fructose, sodium metaperiodate, H₂SO₄, NaOH, NaCl, HCl and CaCl₂ were purchased from Merck (Germany). Other reagents like sodium metaperiodate and resorcinol were purchased from Merck (India) and Loba Chemical (India) respectively. The cellulosic materials such as sawdust, sugarcane bagasse and rice bran were collected from local markets (Savar, Dhaka, Bangladesh).

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Janee S., Saha S., Sharmin S., Hasan A.Q., Zohora U.S., Moni R., Islam M.Z, & Rahman M.S. (2024). Construction and investigation of multi-enzyme immobilized matrix for the production of HFCS. PLOS ONE, 19(2), e0292931.

Publication 2024

α-amylase glucoamylase Corn starch D-fructose sodium metaperiodate H2SO4 NaOH NaCl HCl CaCl2 resorcinol

Corresponding Organization : BRAC University

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Variable analysis

independent variables

Enzymes (α-amylase, glucoamylase, and glucose isomerase)
Cellulosic materials (sawdust, sugarcane bagasse, and rice bran)

dependent variables

Not explicitly mentioned

control variables

Chemicals and reagents (corn starch, D-fructose, sodium metaperiodate, H2SO4, NaOH, NaCl, HCl, CaCl2, sodium metaperiodate, and resorcinol)

controls

No positive or negative controls were mentioned in the provided information.

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