Apoptotic genes (Bax) and anti-apoptotic gene (Bcl-2)13 (link)
 were estimated via using commercially available kits supplied from TOPreal™ qPCR 2X PreMIX (SYBR Green with low ROX) (Cat. # P725, Enzynomics, Korea).
The primer sequences13 (link)
;

Bax: (Size: 109 bp, Accession no. NM_017059.2)

5′-CGAATTGGCGATGAACTGGA-3′ (forward)

5′-CAAACATGTCAGCTGCCACAC-3′ (reverse);

Bcl-2: (Size: 135 bp, Accession no. NM_016993.1)

5′-GACTGAGTACCTGAACCGGCATC-3′ (forward)

5′-CTGAGCAGCGTCTTCAGAGACA-3′ (reverse);

Gene expressions were measured using the below formula and Ct (2–ΔΔCt) (fold change) method:

ΔΔCt = (Cttarget–Ctreference) test sample–(Cttarget–Ctreference) control sample

Finally, considering the primer efficiency value of ~2, the gene expression level was determined as 1–ΔΔCt.