Acetamiprid Insecticide Dose Preparation

All chemicals were purchased from Sigma-Aldrich (Saint Quentin, France). Acetamiprid technical grade insecticide (CAS: 135410-20-7, purity ≥ 98%) was purchased from Sigma Aldrich (France) (Figure 1A) and was dissolved in dimethylsulfoxide (DMSO) to prepare a concentrated stock solution. The stock solution was extemporaneously diluted to obtain a working solution of acetamiprid at 0.01% DMSO. The control consisted of DMSO diluted to a final concentration of 0.01% DMSO in a saline solution. The saline solution contained (in mM): NaCl 208; KCl 3.1; CaCl₂ 5.4; NaHCO₃ 2; sucrose 26; and had a pH of 7.4 [24 (link)].