Statistical analysis was performed, according to the procedures described in previously published papers [9 (link),18 (link),19 (link),20 (link),30 (link),31 (link),32 (link),33 (link),34 (link),35 (link)]. However, the statistical analysis procedures are briefly described as follows: “The images of 2D PAGE gels obtained from the renal proteome fractionation experiments (Groups C-60 and Hg-60) were analyzed with ImageMaster 2D Platinum 7.0 software (GE Healthcare). The analyses with ImageMaster 2D Platinum 7.0 software allowed us to obtain correlations between the images of gels (matching), through equivalence analysis between the protein spots in each 2D PAGE run. This also allows a comparison analysis among the protein spots in terms of distribution, volume, relative intensity, isoelectric point and molecular mass. The results of mercury determinations were expressed as M ± SD, and subjected to Student’s t test and F test to identify any significant differences, using SAS software (version 8). The significance level was set at 5%, that is, p < 0.05”. For protein expression data, statistical analysis was performed using the Protein Lynx Global Server software (version 2.5) (PLGS) of the nanoAcquity UPLC Xevo QTof MS equipment, as described in the previous section.
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