Serum Cytokine Profiling in Mice

Blood was collected from the eyes of the mice and was allowed to clot for at least 30 min before centrifugation for 10 min at 1000 × g. Then, the serum was removed and assayed on a multiplex LUMINEX xMAP MAGPIX instrument (Millipore Corporation). Antibody beads, controls, wash buffer, serum matrix, and standards were prepared for the MILLIPLEX® MAP Kit Mouse Th17 Magnetic Bead Panel (Millipore Corporation) following the manufacturer's instructions [28 (link)]. Concentrations of eight cytokines (IL-6, TNF-α, IL-1β, IL-17A, IL-10, IL-4, IFN-γ, and IL-2) were detected using the Mouse Th17 Magnetic Bead Panel according to its instructions. MAGPIX and xPONENT software were used to read the results.

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Huang Y., Tang J., Cai Z., Zhou K., Chang L., Bai Y, & Ma Y. (2020). Prevotella Induces the Production of Th17 Cells in the Colon of Mice. Journal of Immunology Research, 2020, 9607328.

Publication 2020

LUMINEX xMAP MAGPIX

Antibody Blood Buffer Centrifugation Clot Cytokines Eyes Ifn γ Il 10 Il 17a Il 1β Mouse Serum Th17 Tnf α

Corresponding Organization : Air Force General Hospital PLA

Other organizations : Second Hospital of Shanxi Medical University, Shanxi Medical University

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Concentrations of eight cytokines (IL-6, TNF-α, IL-1β, IL-17A, IL-10, IL-4, IFN-γ, and IL-2)

control variables

Not explicitly mentioned

controls

Controls were prepared for the MILLIPLEX® MAP Kit Mouse Th17 Magnetic Bead Panel (Millipore Corporation) following the manufacturer's instructions.

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