Antigen-coated microspheres were incubated with heat-inactivated sera (37°C, 2 hours). Guinea pig complement (Cedarlane) 1:60 was added (37°C, 20 minutes), then anti-C3 PE-conjugated goat polyclonal IgG (1 µg/mL; MP Biomedicals). C3 deposition was quantified on MAGPIX (Luminex) as detailed above [8 (link)].
Free full text: Click here