Individual tablets of TRUVADA (tenofovir/emtricitabine; Gilead Sciences) or raltegravir (Merck) were crushed into fine powder and manufactured as 5BXL by TestDiet based on previously published 26 (link); 27 (link). HIV-1 RNA was extracted from plasma (Viral RNA Mini Kit, Qiagen). RNA was reverse transcribed and quantitatively detected by real-time PCR using TaqMan® Fast Virus 1-Step PCR kit (ThermoFisher Scientific) and QuantStudio 6 Flex PCR system (Applied Biosystems) (detection limit ≈400 copies/ml)23 (link); 28 (link).
Protocol detail
Quantifying HIV-1 RNA from Crushed ARV Tablets
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Corresponding Organization :
Other organizations : Temple University, University of North Carolina at Chapel Hill, University of Maryland, Baltimore
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Variable analysis
independent variables
- Crushed individual tablets of TRUVADA (tenofovir/emtricitabine; Gilead Sciences)
- Crushed individual tablets of raltegravir (Merck)
- HIV-1 RNA levels in plasma
- Viral RNA Mini Kit (Qiagen) for RNA extraction
- TaqMan® Fast Virus 1-Step PCR kit (ThermoFisher Scientific) for reverse transcription and quantitative PCR detection
- QuantStudio 6 Flex PCR system (Applied Biosystems) for detection
- Detection limit of approximately 400 copies/ml
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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